Hitgen, Inc.

Guansai Liu

Jin Li

Dengfeng Dou

Jinqiao Wan

Zhi Wang

Xing Wang

Yang Chen

Lina Zhong

Yun Mu

Xuemin Cheng

Jian Liu

Dongyan Hu

Qiuxia Chen

Yong Chang

Barry A Morgan

Yanshan Xu

Xincheng Liu

The present invention provides a method of solid-phase synthesis of DNA-encoded compound library. The method includes following steps: a) reacting solid carrier G-1 with linker molecule L-1 to prepare L-G-1; b) reacting DNA with linker molecule L-0 to prepare L-2; c) reacting L-G-1 with L-2 to prepare L-G-2; d) removing protection group of the L-G-2 and obtaining L-G-2-1; e) reacting the L-G-2-1 with synthetic building block and performing DNA encoding; and f) removing the solid carrier and obtaining the DNA-encoded compound library. Compared with the prior art, the present invention can complete post-treatment purification of the reaction only by filtration and irrigation processes for several times. The present invention is simple to operate, can shorten the production cycle of DNA encoded compound library with more than 50%, significantly increases the production efficiency and the unicity as well as the purity of the final products.

Method for solid-phase synthesis of DNA encoded compound library

Growing community of inventors

Chengdu, China

Guansai Liu