Bgi Shenzhen

Bgi-Shenzhen Co., Limited

Guangyi Fan

Hui Fu Jiang

Wenwei Zhang

Handong Li

Chunyu Geng

He Zhang

Jing Yu

Kailong Ma

Hui Jiang

Y Tom Tang

Improved single molecule sequencing methods, compositions, and devices, are provided. In a first aspect, the present invention provides a multi-pass method of sequencing a target sequence using nanopore sequencing, the method comprising: i) providing a non-naturally occurring concatemer nucleic acid molecule comprising a plurality of copies of the target sequence; ii) nanopore sequencing at least three copies of the target sequence in the concatemer, thereby obtaining a multi-pass sequence dataset, wherein the multi-pass sequence dataset comprises target sequence datasets for the at least three copies of the target sequence; and iii) using the multi-pass sequence dataset to determine the target sequence.

Multi-pass sequencing

Growing community of inventors

Shenzhen, China

Guangyi Fan