Applied Research Systems Ars Holding N.v.

Serono Diagnostics Ltd.

Other

Ares-serono Research & Development Limited Partnership

Grenville Arthur Robinson

Gordon C Forrest

Simon J Rattle

Hugh A Hill

John W Attridge

Phelim Brinley Daniels

Martyn Omar Rowlands

Michael Anthony Hobbs

Julie Karen Deacon

Colin Andrew Love

Aileen Margaret Thomson

John Richard Calvert

Gerald John Allen

Alan Derek Cookson

Rosemary Ann Drake

Janys Elizabeth Fletcher

John G Hurrell

Craig George Sawyer

Madlyn D Denyer

A device for reconstituting liquid for medical use by bringing together a first liquid medium contained in a first vessel in the form of a cartridge () and a second medium, such as a drug in solid form, contained in a second vessel in the form of a vial (), the device () comprising means () for supporting the first and second vessels, and a movable operating member () for applying a force to cause the first liquid medium to be delivered at a controlled rate from the first vessel into the second vessel. The first and second vessels may be provided in a pack () having liquid transfer means in the form of a needle ().

Reconstituting device for injectable medication

A device for reconstituting liquid for medical use by bringing together a first liquid medium contained in a first vessel in the form of a cartridge ( ) and a second medium, such as a drug in solid form, contained in a second vessel in the form of a vial ( ), the device ( ) comprising means ( ) for supporting the first and second vessels, and a movable operating member ( ) for applying a force to cause the first liquid medium to be delivered at a controlled rate from the first vessel into the second vessel. The first and second vessels may be provided in a pack ( ) having liquid transfer means in the form of a needle ( ).

A method of assaying for a ligand in a sample which includes incubating, simultaneously or in any desired sequence,

Wood's anomaly assay technique and kit

A method of assay for a ligand in a sample is described in which calibration occurs within the assay. This is achieved utilizing a measurement region and one or more calibration regions. In at least one of the calibration regions a non-zero signal results, either because of the presence of a calibration reagent or as a result of a binding reaction analogous to that which takes place in the measurement region.

Capillary-fill biosensor device comprising a calibration zone

Sensor devices for use in assaying for a substance selected from (i) enzymes capable of producing a change in their environment as a result of catalytic reaction with a substrate and (ii) substrates for such enzymes is described, the devices comprising an optical waveguide having immobilized directly or indirectly on a discrete region ('the measurement region') of one longitudinal surface thereof a species whose optical properties change as a result of the aforementioned change in its environment together with the member of an enzyme substrate/enzyme pair complementary to the substance under assay. Methods of assay using such devices are also described.

Optical sensor for enzyme and enzyme substrates

Sensor device for sandwich assay

Spectrophotometric techniques

A method of assay for a ligand in a sample is described in which calibration occurs within the assay. This is achieved utilizing a measurement region and one ore more calibration regions. In at least one of the calibration regions a non-zero signal results, either because of the presence of a calibration reagent or as a result of a binding reaction analogous to that which takes place in the measurement region.

Method of assay having calibration within the assay

Determination of substrate of coenzyme requiring enzyme

Method of assay normalized with non-antigen calibrator

An electrochemical specific binding assay of a ligand (e.g., antigen, hapten or antibody) wherein at least one of the components is enzyme-labelled, and which includes the step of determining the extent to which the transfer of electrons between the enzyme substrate and an electrode, associated with the substrate reaction, is perturbed by complex formation or by displacement of any ligand complex relative to unbound enzyme-labelled component. The electron transfer is aided by electron-transfer mediators which can accept electrons from the enzyme and donate them to the electrode or vice versa (e.g. ferrocene) or by electron-transfer promoters which retain the enzyme in close proximity with the electrode without themselves taking up a formal charge. The electrochemical apparatus will typically comprise two or three electrodes, including one working electrode onto which components may advantageously be immobilized. The use of direct electrochemical measurement of the enzyme label avoids the errors and inconvenience of the known indirect measurement techniques.

Methods of assay

A method of assaying a ligand in a sample which method includes the steps of contacting the sample with components comprising

Use of charge transfer complexes in assay

In its broadest aspect, the present invention provides a method of effecting an electrochemical specific-binding assay of a ligand, either qualitatively or quantitatively, in an apparatus comprising at leasts one electrode, in which method a labelled component of the assay medium is, at least in part, magnetically held in the vicinity of the electrode. The electrochemical assay method may include a separation step, whereby bound label may be separated from free label in the assay medium. Preferably the electrochemical assay method will include the step of determining a perturbation in an electrochemical characteristic of components of the assay medium associated with a ligand complexing reaction. Such an assay method is applicable to, for example, antibodies and antigens and suitable labels include redox centers, enzyme labels in the presence of an electron-transfer mediator and electron-transfer mediator labels in the presence of an electron donor or acceptor.

Method of assay employing a magnetic electrode

A ligand in a sample can be assayed using electrochemical apparatus containing a working electrode and components comprising:

Electrochemical methods of assay

In its broadest aspect, the present invention provides a method of assaying a ligand in a sample which comprises contacting the sample with a predetermined quantity of a specific binding partner to the ligand, said specific binding partner being immobilized on the effective gate electrode of a field effect transistor, and determining whether (and, if desired, the extent to which) an appropriate transistor characteristic is changed as a result of complex formation between the ligand and the specific binding partner.

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London, United Kingdom

Grenville Arthur Robinson