Cornell Research Foundation Inc.

Other

Gregory T Baxter

Michael L Shuler

Scott Meyers

Aaron Sin

Robert Andrew Harrison

Richard Kuo

Sandip Tiwari

Michael Simms Shuler

David Epel

A cell culture device comprising a microscale chamber dimensioned to maintain a cell under conditions that give rise to a value for at least one pharmacokinetic parameter in vitro that is comparable to a value for the same at least one pharmacokinetic parameter obtained with respect to the same type of cell in vivo, wherein the microscale chamber is configured for flow of culture medium, and wherein the at least one pharmacokinetic parameter is selected from tissue size ratio, tissue to blood volume ratio, drug residence time, flow rate, circulatory transit time, liquid residence time, and liquid to cell ratio.

Devices and methods for pharmacokinetic-based cell culture system

A cell culture method using an in vitro microscale cell culture device having a first microscale chamber containing cells attached to an inner surface of the chamber and cell culture medium in contact with the cells. The cells are maintained in culture in the in vitro microscale cell culture device while flowing culture medium through the first chamber at a controlled rate to provide a liquid residence time value for the cells in the first chamber of the in vitro microscale cell culture device that is comparable to a liquid residence time value obtained with respect to comparable cells in vivo, such that metabolism by the cells in culture in the in vitro microscale cell culture device is comparable to metabolism by comparable cells in vivo.

Devices, in vitro cell cultures, systems, and methods are provided for microscale cell culture analogous (CCA) device.

A gated metal oxide semiconductor field effect transistor (MOSFET) gain cell is formed with a flow channel for molecule flow. The flow channel is formed under the gate, and between a source and drain of the transistor. The molecule flow modulates a gain of the transistor. Current flowing between the source and drain is representative of charges on the molecules flowing through the flow channel. A plurality of individually addressable gain cells are coupled between chambers containing samples to measure charges on molecules in the samples passing through the gain cells.

Electronic gain cell based charge sensor

Nitric oxide synthase and nitric-oxide-related bioactivity are shown to be necessary and sufficient for activation of an oocyte during the process of fertilization. Methods are provided for the modulation of oocyte activation through modulation of nitric oxide levels. Oocyte activation is enhanced by the addition of nitric oxide synthase, nitric oxide synthase activators, nitric oxide donors, or other compounds that increase the nitric oxide levels of the oocyte during fertilization. The administration of NOS inhibitors is used to block oocyte activation, where contraception is desired.

Methods for modulation of oocyte activation

A micro-machined nuclear transfer array (NTA) provides high-throughput transfer of nuclei between two cells. Donor cells containing the nuclei to be transferred are placed in microwells positioned adjacent to microwells containing the recipient biological cell. The microwells are contained within an upper chamber patterned with parallel rows of microwells of the diameter of the cell of interest. An injection port is formed in the bottom of the microwells through which nuclei may pass during transfer. The NTA also contains a lower chamber having a second array of capture wells of similar dimension and in register with the upper chamber for receiving the nuclei removed from the cell in the upper chamber. Nuclei are removed from the donor cells in the upper chamber, transferred to capture wells in the lower chamber, and inserted into the recipient biological cells of the upper chamber. The upper tray moves relative to the lower chamber so that the nuclei in the capture well are transferred to the recipient biological cell to complete the nuclear transfer process.

Growing community of inventors

Salinas, CA, United States of America

Gregory T Baxter