Danisco US Inc.

The Goodyear & Tire Rubber Company

Genencor International, Inc.

E.i. Dupont De Nemours and Company

Other

Eastman-kodak Company

Genencor Intl., Inc.

The Procter Gamble Company

Gregory Marshall Whited

Joseph Charles McAuliffe

Marguerite A Cervin

Gopal K Chotani

Karl John Sanford

Fernando Valle

Andrei Miasnikov

Caroline M Peres

Frank James Feher

Aaron Scott Puhala

Richard Ray Bott

Michael C Miller

Sharon Loretta Haynie

Anthony R Calabria

Walter Weyler

Jeff P Pucci

Lisa Anne Laffend

Carol Marie Fioresi

Gregory Scot Miracle

Jeffrey John Scheibel

Ayrookaran J Poulose

Brian E Jones

Philippe Soucaille

James T Kellis, Jr

Derek H Wells

Donald Eugene Trimbur

Charles T Goodhue

Hiroshi Oh

Bradley R Kelemen

Neelam S Amin

Sandra W Ramer

Anthony Arthur Gatenby

Mark Scott Payne

Manoj N Kumar

Vasantha Nagarajan

Stephen K Picataggio

Olga V Selifonova

Richard La Duca

Timothy A Sabo

Mark Emptage

Erin L Spring

Wyatt Charles Smith

Ilana S Aldor

Christopher Lee Rife

Frank A Pettrone

Rasmus B Jensen

Donald E Ward, Ii

Marc Eric Gustwiller

Klaus Liebeton

Edward M Concar

Matthew G Boston

Charles Edwin Nakamura

Ramesh V Nair

Robert Seemayer

Matthew Grant Boston

Maria R Diaz-Torres

Matthew W Chase

Theresa C Paulson

Christopher C Barnett

Ben A Bulthuis

Amy Kuang-Hua Hsu

Patrick J Grisdale

Erin L Webster

Juergen Wiegel

Markus Seyfried

Maguerite A Cervin

Richard D La Reau

Manoj Kumar

Michael Charles Miller

Anthony Rudolf Calabria

Joseph C Mcauliffe

Richard D Lareau

Stephen Kenneth Picataggio

Brian Edward Jones

Timothy Alan Sabo

Erin Lynne Webster

Christopher Barnett

Neelem S Amin

Donald E Ward Ii

The invention features methods for producing isoprene from cultured cells wherein the cells in the stationary phase. The invention also provides compositions that include these cultured cells and/or increased amount of isoprene. The invention also provides for systems that include a non-flammable concentration of isoprene in the gas phase. Additionally, the invention provides isoprene compositions, such as compositions with increased amount of isoprene or increased purity.

Compositions and methods for producing isoprene free of c5 hydrocarbons under decoupling conditions and/or safe operating ranges

The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.

Compositions and methods for producing isoprene

Compositions and methods for producing isoprene free of C5 hydrocarbons under decoupling conditions and/or safe operating ranges

It has been found that certain cells in culture can convert more than about 0.002 percent of the carbon available in the cell culture medium into isoprene. These cells have a heterologous nucleic acid that (i) encodes an isoprene synthase polypeptide and (ii) is operably linked to a promoter. The isoprene produced in such a cultured medium can then be recovered and polymerized into synthetic rubbers and other useful polymeric materials. The synthetic isoprene containing polymers of this invention offer the benefit of being verifiable as to being derived from non-petrochemical based resources. They can also be analytically distinguished from rubbers that come from natural sources. The present invention more specifically discloses a polyisoprene polymer which is comprised of repeat units that are derived from isoprene monomer, wherein the polyisoprene polymer has &#x3b4;C value of greater than &#x2212;22%.

Polymers of isoprene from renewable resources

The present invention provides methods and compositions comprising at least one perhydrolase enzyme for cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions for generation of peracids. The present invention finds particular use in applications involving cleaning, bleaching and disinfecting.

Perhydrolase

The invention features methods for producing isoprene from cultured cells wherein the cells in the stationary phase. The invention also provides compositions that include these cultured cells and/or increased amount of isoprene. The invention also provides for systems that include a nonflammable concentration of isoprene in the gas phase. Additionally, the invention provides isoprene compositions, such as compositions with increased amount of isoprene or increased purity.

The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells. The invention provides isoprene compositions, such as compositions with increased amount of isoprene or increased purity. Additionally, the invention provides methods of producing isoprene by culturing cells under conditions suitable for isoprene production while maintaining cell viability and/or metabolic activity.

Systems using cell culture for production of isoprene

It has been found that certain cells in culture can convert more than about 0.002 percent of the carbon available in the cell culture medium into isoprene. These cells have a heterologous nucleic acid that (i) encodes an isoprene synthase polypeptide and (ii) is operably linked to a promoter. The isoprene produced in such a cultured medium can then be recovered and polymerized into synthetic rubbers and other useful polymeric materials. The synthetic isoprene containing polymers of this invention offer the benefit of being verifiable as to being derived from non-petrochemical based resources. They can also be analytically distinguished from rubbers that come from natural sources. The present invention more specifically discloses a polyisoprene polymer which is comprised of repeat units that are derived from isoprene monomer, wherein the polyisoprene polymer has &#x3b4;C value of greater than &#x2212;22&#x2030;.

Polymers of isropene from renewable resources

The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.

Isoprene synthase variants for improved microbial production of isoprene

The invention provides methods, compositions, and kits for removal of biofilms from surfaces. The methods described herein comprise simultaneous or sequential application of a perhydrolase enzyme and a mixture of other enzymes, such as proteases, glucanases, esterases, mannanases, phospholipases, cellulases, and/or amylases, to a biofilm on a surface, to effect removal of the biofilm.

Enzymatic prevention and control of biofilm

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Belmont, CA, United States of America

Gregory Marshall Whited