Sigma-aldrich Co. LLC

Mbio Diagnostics, Inc.

Gregory M Husar

Michael J Lochhead

Wolfgang A Pieken

Andreas Wolter

Michael Leuck

David P Sebesta

Sarah Rachel Bickman

Hallie A Latham-Timmons

John Pilon

A method for performing a high dynamic range immunoassay includes (a) measuring a first signal from a sandwich immunoassay to detect a target analyte in a fluidic sample in a fluidic channel and a second signal from a competitive immunoassay associated with the target analyte in the same fluidic sample in the fluidic channel, and (b) determining ratio of the first signal to the second signal to provide a measure of concentration of the target analyte in the fluidic sample, wherein the measure is applicable to a high dynamic range of concentrations of the target analyte.

Methods and devices for performing high dynamic range immunoassays

This invention discloses a novel method for immobilizing molecules to a support. Specifically, this invention discloses a method of immobilizing derivatized biomolecules, such as oligonucleotides, using cycloaddition reactions, such as the Diels-Alder reaction. Included in this invention are the novel immobilized biomolecules that can be prepared according to the method of this invention.

Method for immobilizing oligonucleotides employing the cycloaddition bioconjugation method

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Longmont, CO, United States of America

Gregory M Husar