The Clorox Company

Genencor, Inc.

Gregory L Gray

Scott Douglas Power

Ayrookaran J Poulose

Richard J Wiersema

Anna G Stanislowski

Ayrookaram J Poulose

A substantially enzymatically pure hydrolase is provided which is secreted by and isolatable from Pseudomonas mendocina ATCC 53552. Cloning the gene expressing the hydrolase into a suitable expression vector and culturing, such as fermenting the E. coli strain JM101 harboring a plasmid designated pSNtacII, has been found to provide surprisingly high yields of the hydrolase.

Lipase from Pseudomonas mendocina having cutinase activity

An enzymatic perhydrolysis system, useful for bleaching, has a novel enzyme, a substrate, and a source of hydrogen peroxide, and provides in situ formation of peracid in aqueous solution. The substrate is selected for enzyme catalyzed reaction, and preferably is an acylglycerol with two or three fatty acid chains. The enzyme is hydrolytically and perhydrolytically active even in the presence of anionic surfactants, has lipase activity, and is isolatable from Pseudomonas putida ATCC 53552.

Enzymatic peracid bleaching system

Growing community of inventors

South San Francisco, CA, United States of America

Gregory L Gray