Pacific Biosciences of California, Inc.

Gregory Kearns

Stephen Wilson Turner

Pezhman Monadgemi

Mathieu Foquet

Ron Kuse

Drew Martinez

Jeremy Gray

Natasha Popovich

Ronald L Cicero

Stephen P Dudek

Robert Sebra

Methods, compositions and arrays for non-random loading of single analyte molecules into array structures are provided. Arrays of confined regions are produced wherein each confined region comprises a single island within the confined region. The island can be selectively functionalized with a coupling agent to couple a single molecule of interest within the confined region.

Nanoscale apertures having islands of functionality

Methods, substrates, and devices related to arrays of optical confinements having surfaces with high levels of bias. Substrates having transparent or silica based portions and opaque or reflective portions are treated with 1) a selective passivating agent, that selectively coats the opaque or reflective regions, 2) a functionalizing agent such as a coupling agent, and 3) a selective removal agent, which selectively removes functionalizing agent from the passivated opaque or reflective surfaces.

Selectively functionalized arrays

Growing community of inventors

San Mateo, CA, United States of America

Gregory Kearns