Tecan Trading Ag

Netbio, Inc.

Gamera Bioscience Corp.

Ande Corporation

Radisens Diagnostics Limited

Covaris, Inc.

Tecan Boston

Gregory John Kellogg

Bruce L Carvalho

Stephen G Kieffer-Higgins

Eugene Tan

Heung Chuan Lam

Alec Mian

Norman F Sheppard, Jr

Richard F Selden

John A Wright

Mikayla Kob

Shari Ommert

John P Willis

Ulrich Hans Thomann

Gene A Davis

Laura L Chapman

Ted Minior

Sarah D Oeltjen

Valery Leonidovich Bogdanov

Heidi Susanne Giese

James A Laugharn, Jr

David C Duffy

Christina M Feakes

Timothy J Johnson

David Doolan

Donal Cronin

Mona D Jensen

Todd Edward Arnold

Keith Morneau

Eric Schilling

Hsin Chiang Lin

Norman F Sheppard

Kevin E Noonan

Hueng Chuan Lam

Hsin-Chiang Lin

Charles Able

Andrea Pierce

Hans Thomann Ulrich

Heidi S Giese

Heung C Lam

The present disclosure provides fully integrated microfluidic systems to perform nucleic acid analysis. These processes include sample collection, nucleic acid extraction and purification, amplification, sequencing, and separation and detection. The present disclosure also provides optical detection systems and methods for separation and detection of biological molecules. In particular, the various aspects of the invention enable the simultaneous separation and detection of a plurality of biological molecules, typically fluorescent dye-labeled nucleic acids, within one or a plurality of microfluidic chambers or channels. The nucleic acids can be labeled with at least 6 dyes, each having a unique peak emission wavelength. The present systems and methods are particularly useful for DNA fragment sizing applications such as human identification by genetic fingerprinting and DNA sequencing applications such as clinical diagnostics.

Integrated nucleic acid analysis

A microfluidic system for processing biological samples comprising a holding chamber adapted for holding a fluid and to be rotated on a platform, said holding chamber comprising an outlet through which fluid flow is controlled by an acceleration-primed valve system, wherein the acceleration-primed valve system comprises a capillary valve and an outlet channel. The invention provides a novel valving system, which retains fluids at low angular velocities, removes the need for hydrophilic surfaces, minimises disc real-estate and optimises certain microfluidic processes done in the holding chamber.

Acceleration-primed valving system for centrifugal microfluidics

The invention provides methods and systems for ruggedizing a nucleic acid analyzing apparatus. The ruggedized apparatus can be used reliably and effectively in uncontrolled environments, such as, for example at a crime scene to collect and analyze forensic data, as well as in semi-controlled environments, such as, for example at a point of care location.

Ruggedized apparatus for analysis of nucleic acid and proteins

The invention relates to a microfluidic system for processing biological samples comprising a holding chamber adapted for holding a fluid and to be rotated on a platform, said holding chamber comprising an outlet through which fluid flow is controlled by an acceleration-primed valve system, wherein the acceleration-primed valve system comprises a capillary valve and an outlet channel. The invention provides a novel valving system, which retains fluids at low angular velocities, removes the need for hydrophilic surfaces, minimizes disc real-estate and optimizes certain microfluidic processes done in the holding chamber.

Valving system for use in centrifugal microfluidic platforms

A treatment vessel may allow a user, or automated system, to manipulate sample material within a treatment area during processing (e.g., focused acoustic treatment), as well as subject the sample material to a staged processing protocol. The vessel may include openings for receiving/discharging the sample material. Walls within the vessel may be movable between various positions, to permit or obstruct flow of sample material into or out from a treatment area. The wall(s) may push the sample material within the vessel, as well as adjust pressure levels within the treatment area. In some embodiments, an acoustic treatment system may include a flexible coupling medium that may be deformed toward the vessel upon an application of suitable pressure thereto. When the medium presses up against the vessel, defects (e.g., particles, bubbles, interfaces, etc.) that may otherwise be present along the acoustic wave path may be reduced.

Methods and apparatuses for acoustic treatment of samples

Integrated systems for the multiplexed amplification and detection of six and greater dye labeled fragments

A fast, multiplexed PCR system is described that can rapidly generate amplified nucleic acid products, for example, a full STR profile, from a target nucleic acid. Such systems include, for example, microfluidic biochips and a custom built thermal cycler, which are also described. The resulting STR profiles can satisfy forensic guidelines for signal strength, inter-loci peak height balance, heterozygous peak height ratio, incomplete non-template nucleotide addition, and stutter.

Methods for rapid multiplexed amplification of target nucleic acids

This invention relates to methods and apparatus for performing microanalytic and microsynthetic analyses and procedures. The invention provides a microsystem platform and a micromanipulation device for manipulating the platform that utilizes the centripetal force resulting from rotation of the platform to motivate fluid movement through microchannels. These assays may be performed for a variety of purposes, including but not limited to screening of drug candidate compounds, life sciences research, and clinical and molecular diagnostics. Methods specific for the apparatus of the invention for performing any of a wide variety of microanalytical or microsynthetic processes are provided.

Microfluidics devices and methods of diluting samples and reagents

This invention relates to methods and apparatus for performing microanalytic and microsynthetic analyses and procedures. The invention provides a microsystem platform and a micromanipulation device for manipulating the platform that utilizes the centripetal force resulting from rotation of the platform to motivate fluid movement through microchannels. The microsystem platforms of the invention are provided having arrays of thermal control regions, wherein fluid applied to the platform can be placed at a temperature and maintained at that temperature for a time that is dependent on the path length of the channel in the region, the cross-section dimension of the channel, and the rotational speed of the platform. Methods specific for the apparatus of the invention for performing any of a wide variety of microanalytical or microsynthetic processes are provided.

Centripetally-motivated microfluidics system for performing in vitro hybridization and amplification of nucleic acids

Growing community of inventors

Cambridge, MA, United States of America

Gregory John Kellogg