Life Technologies Corporation

Avidbiotics Corporation

Gregory Govoni

Manohar R Furtado

Maxim G Brevnov

Dean M Scholl

Dana Gebhart

Kevin Hacker

Johnie Young

Mariela Cuadras

Nikolay Sergeev

James C Nurse

David W Martin, Jr

Steven R Williams

Elena Bolchakova

Jim Nurse

Elena Bolchacova

Devices and methods for detecting microbial contaminants, such as bacteria and fungi, in fluids such as drinking water, pharmaceutical solutions and tissue culture media are provided. More particularly, provided are filtration devices for capture and processing of microorganisms from fluids, and improved methods for recovery, lysis and detection of microorganisms based on a combination of physical disruption with small beads and lysis solutions.

Device for capture and lysis of microorganisms from liquids and methods of use thereof

This disclosure relates to the discovery and isolation of the entire cluster of genes encoding R-type high molecular weight bacteriocins that specifically killbacteria, dangerous pathogens. Also disclosed are methods of producing the R-type bacteriocins in innocuous aerobic producer cells. Disclosed also are small, non-ORF1374 receptor binding domains (RBDs), which are incorporated into diffocins to form engineered or variant diffocins having altered killing spectra. Variant diffocins provided herein may include a heterologous RBD and its cognate base plate attachment region (BPAR), or a fused BPAR. This invention offers a potent bactericidal agent with increased thermal and pH stability, and methods for producing it, in order to kill selectivelybacteria in the environment of the gastrointestinal tract where they can cause great harm and even death of the infected patient or farm animal.

Diffocins and methods of use thereof

Device for capture and lysis of microorganisms from liquids

This disclosure relates to the discovery and isolation of the entire cluster of genes encoding R-type high molecular weight bacteriocins that specifically killbacteria, dangerous human pathogens. Also disclosed are methods of producing the R-type bacteriocins in innocuous producer cells that, unlike, do not die in the presence of oxygen. Disclosed also is the specific gene of the isolated gene cluster that determines the killing spectrum of the R-type bacteriocin and the demonstration that the killing spectra of diffocins can be altered by engineering orf1374 of the diffocin genetic locus. This invention offers a potent bactericidal agent and a means to make it in order to kill selectivelybacteria in the environment of the gastrointestinal tract where they can cause great harm and even death of the infected patient or farm animal.

Diffocin and methods of use thereof

Growing community of inventors

San Carlos, CA, United States of America

Gregory Govoni