Berkeley Lights, Inc.

University of California

Bruker Cellular Analysis, Inc.

Gregory G Lavieu

Kristin G Beaumont

Randall D Lowe, Jr

Volker L S Kurz

Annamaria Mocciaro

J Tanner Nevill

Mark P White

Alexander J Mastroianni

Aathavan Karunakaran

Xiaohua Wang

Xiao Guan Radstrom

Magali Soumillon

Jason M McEwen

Ravi K Ramenani

Jian Gong

John A Tenney

Kevin T Chapman

I-Jong Lin

Peter J Beemiller

Patricia A Dyck

Troy A Lionberger

Alexander Marson

Gang F Wang

Theodore Lee Roth

Guido K Stadler

Yelena Bronevetsky

Hayley M Bennett

Kevin D Loutherback

Matthew E Fowler

Anupam Singhal

Phillip J M Elms

Jr Lowe Randall D

Jr Randall D Lowe

In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to provide improved or altered performance with biomaterials.

Covalently modified surfaces, kits, and methods of preparation and use

Methods are described herein for isolating clonal populations of T cells having a defined genetic modification. The methods are performed, at least in part, in a microfluidic device comprising one or more sequestration pens. The methods include the steps of: maintaining individual T cells (or precursors thereof) that have undergone a genomic editing process in corresponding sequestration pens of a microfluidic device; expanding the T cells into respective clonal populations of T cells; detecting, in one or more T cells of each clonal population, the absence of a cell surface marker that was present in the individual T cells (or precursors thereof); and detecting, in one or more T cells of each clonal population, the presence of a first nucleic acid sequence that is indicative of the presence of an on-target genome edit in the clonal population of T cells. Also described are compositions comprising one or more clonal populations of T cells isolated according to the methods disclosed herein.

Methods for selection and generation of genome edited T cells

In situ-generated microfluidic capture structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. Microfluidic capture structures may be advantageously used for assays performed within the microfluidic environment, providing flexibility in assaying micro-objects such as biological cells. Assay reagents and analytes may be incorporated within the microfluidic capture structures.

In situ-generated microfluidic assay structures, related kits, and methods of use thereof

The present disclosure provides methods of preparing tumor infiltrating cells engineered to express a pro-inflammatory polypeptide. The pro-inflammatory polypeptide is expressed from the tumor infiltrating cell to counter a generally immunosuppressive state in and around tumors resulting from an imbalance between the number and activation state of immune effector cells versus those of suppressor cells. Delivering the proinflammatory polypeptide via expression from the TICs, as distinct from systemic administration, reduces side effects from increased inflammation at sides remote from a tumor to be treated.

Tumor infiltrating cells engineered to express a pro-inflammatory polypeptide

Methods, systems and kits are described herein for detecting the results of an assay. In particular, the methods, systems and devices of the present disclosure rely on a difference between the diffusion rates of a reporter molecule and an analyte of interest in order to quantify an amount of analyte in a microfluidic device. The analyte may be a secreted product of a biological micro-object.

Methods, systems and kits for in-pen assays

Methods are described herein for isolating clonal populations of cells having a defined genetic modification. The methods are performed, at least in part, in a microfluidic device comprising one or more sequestration pens. The methods include the steps of: maintaining individual cells (or precursors thereof) that have undergone a genomic editing process in corresponding sequestration pens of a microfluidic device; expanding the individual cells into respective clonal populations of cells; and detecting, in one or more cells of each clonal population, the presence of a first nucleic acid sequence that is indicative of the presence of an on-target genome edit in the clonal population of cells. Also described are methods of performing genome editing within a microfluidic device, and compositions comprising one or more clonal populations of cells generated according to the methods disclosed herein.

Methods, systems and devices for selection and generation of genome edited clones

Growing community of inventors

Vitry sur Seine, France

Gregory G Lavieu