Mcgill University

Basf Plant Science Gmbh

Dna Landmarks Inc.

Gregory G Brown

Benoit S Landry

Wing Cheung

Natasa Formanova

Charles Dendy

Fang-Ming Lai

Jin Hua

Martine Jean

Hua Jin

Martin LaForest

The present invention includes nuclear fertility restorer genes, proteins encoded by those genes and transgenic plants and plant cells containing those genes. More particularly, the nuclear fertility restorer genes can be used to restore fertility in cytoplasmic male-sterile plants such as. Preferably, the nuclear fertility restorer genes are used with the Ogura (ogu) CMS system in

Nuclear fertility restorer genes and methods of use in plants

The present invention includes nuclear fertility restorer genes, proteins encoded by those genes and transgenic plants and plant cells containing those genes. More particularly, the nuclear fertility restorer genes can be used to restore fertility in cytoplasmic male-sterile plants such asPreferably, the nuclear fertility restorer genes are used with the Ogura (ogu) CMS system in

The present invention relates to methods for enhancement of naturally occurring cytoplastic male sterility and for restoration of male fertility and uses thereof in hybrid crop production. There is also disclosed a method for restoration of male fertility to cytoplasmic male sterile plants; which comprises the steps of: a) introducing into the nucleus of a plant cell a gene construct essentially consisting of a sequence encoding a mitochondrial transit peptide fused upstream of and in frame with an edited form of a normal mitochondrial gene that is co-transcribed with an usual CMS-associated mitochondrial gene; b) selecting for plant cells that have acquired the gene construct in step a); and c) inducing regeneration of selected plant cells to produce a mature plant.

Method for enhancement of naturally occurring cytoplasmic male sterility and for restoration of male sterility and for restoration of male fertility and uses thereof in hybrid crop production

The present invention relates to methods for reducing pollen production in plants. In particular, the present invention relates to a method of reducing pollen production in plants, which comprises the steps of: a) introducing into the nucleus of a plant cell a gene construct essentially consisting of a developmentally regulated promoter driving expression of a sequence encoding a mitochondrial transit peptide fused upstream of and in frame with an edited form of the orf224 gene ofmitochondria, wherein said promoter is expressed in mid- to late-stage stamen development; b) selecting for plant cells that have acquired the gene construct in step a); and c) inducing regeneration of selected plant cells to produce a mature plant with reduced pollen production.

Method for enhancement of naturally occurring cytoplasmic male sterility and for restoration of male fertility and uses thereof in hybrid crop production

The instant invention provides a method for detecting the presence of a restorer gene in the nuclear genomic DNA of a Brassica plant comprising the use of a probe/primer comprising the sequence of the Brassica glyceraldehyde-3-phosphate dehydrogenase (SEQ ID NO: 1) or a sufficient hybridizing fragment thereof.

Glyceraldehyde-3-phosphate dehydrogenase and nuclear restoration of cytoplasmic male sterility

The present invention relates to methods for enhancement of naturally occurring cytoplasmic male sterility and for restoration of male fertility and uses thereof in hybrid crop production. There is also disclosed a method for restoration of male fertility to cytoplasmic male sterile plants; which comprises the steps of: a) introducing into the nucleus of a plant cell a gene construct essentially consisting of a sequence encoding a mitochondrial transit peptide fused upstream of and in frame with an edited form of a normal mitochondrial gene that is co-transcribed with an unusual CMS-associated mitochondrial gene; b) selecting for plant cells that have acquired the gene construct in step a); and c) inducing regeneration of selected plant cells to produce a mature plant.

Growing community of inventors

Montreal, Canada

Gregory G Brown