Abbott Laboratories Corporation

Gregory F Okasinski

Verlyn G Schaefer

Richard R Lesniewski

Peter J DeVries

Joseph L Meuth

Thomas S Suhar

Berry S Mellovitz

This invention provides a novel mammalian expression system that is capable of generating high levels of expressed hepatitis C virus (HCV) proteins which have previously proved difficult to express due to their non-secretory properties. In particular, the invention provides a plasmid for the expression of the HCV second envelope protein (E2) designated p577. This plasmid encodes a recombinant protein comprising the immunoglobulin signal peptide and amino acids 388-664 of the HCV E2 glycoprotein. This unique expression system produces high levels of HCV proteins that are properly processed, glycosylated, and folded.

Hepatitis C virus second envelope (HCV-E2) glycoprotein expression system

DNA encoding human a erythropoietin analog

This invention encompasses an eukaryotic expression vector constructed from a prokaryotic plasmid by inserting into the prokaryotic plasmid a mediator sequence and a promoter/regulatory sequence with a restriction endonuclease site for inserting a gene to be expressed. The gene to be expressed is inserted into the restriction endonuclease site in the promoter/regulatory sequence and the resulting plasmid is in turn used to transform a eukaryotic cell. The gene is expressed with or without viral mediation.

HSV helper virus independent vector

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Wadsworth, IL, United States of America

Gregory F Okasinski