University of Manitoba

Smart Autonomous Solutions, Inc.

Gregory E Bridges

Douglas John Thomson

Azita Fazelkhah

Nicholas Durham

Elham Salimi

Samaneh Afshar Delkhah

A dual light source lens-free dielectrophoresis (DEP) flow cytometer for massively parallel single cell analysis. Each cells dielectric is inferred from measuring their altitude and subsequently velocity change due to DEP actuation in a microfluidic channel. Dual LED sources facilitate velocity measurement by producing two shadows for each cell passing through the channel. These shadows are detected using a linear optical array detector. Massively parallel analysis is possible as each pixel of the detector can independently analyze the passing cells. The DEP cytometer is composed of simple modular components and has the potential to be scaled to achieve a significantly high throughput label-free single-cell analyzer.

Parallel single cell lens free optical dielectrophoresis cytometer

The system () comprises a sensor () 90&#xd7;90&#xd7;90&#xd7;30 mm as an electromagnetic microwave cavity () with a coupler () with a wire () and an antenna (). Cavity () produces a response signal () in response to an interrogation signal () from interrogator (). Sensor () is coupled to a structure () to allow a strain to alter the resonance properties. 3.6 GHz is used with a detection of a 2.5 kHz change. If not temperature via strain is detected a mechanical amplifier is used with cavity () for temperature compensation. Continuous or intermittent narrowband signals are used as interrogation signals (). Used with bridges for structural health monitoring. Also for aircrafts, dams, buildings, vehicles.

Measuring strain in a structure using a sensor having an electromagnetic resonator

Growing community of inventors

Winnipeg, Canada

Gregory E Bridges