Sigma-aldrich Co. LLC

Other

Sangamo Biosciences, Inc.

Sigma-aldvich Co. LLC

Sangamo Therapeutics, Inc.

Gregory D Davis

Fuqiang Chen

Xiao Ding

Yongmei Feng

Timothy Seebeck

Michael C Holmes

Philip D Gregory

Paul H Hanifl

Carol A Kreader

Jeff Steffens

Tim Moran

Vikas B Palhan

Xiaoxia Cui

Qiaohua Kang

Shondra M Pruett-Miller

Edward J Weinstein

Scott W Knight

David Beck

Vikas Palhan

Engineered Cas9 systems that utilize alternate protospacer adjacent motifs for target DNA binding, nucleic acids encoding the engineered Cas9 systems, and methods of using the engineered Cas9 systems for modifying target chromosomal sequences in eukaryotic cells.

Engineered CAS9 systems for eukaryotic genome modification

Compositions and methods for using nucleosome interacting protein domains to increase accessibility of programmable DNA modification proteins to target chromosomal sequences, thereby increasing efficiency of targeted genome/epigenetic modification in eukaryotic cells.

Using nucleosome interacting protein domains to enhance targeted genome modification

The present disclosure provides reagents and methods for molecular proximity detection of specific endogenous nucleic acids in situ using RNA-guided nucleic acid binding proteins.

Methods and reagents for molecular proximity detection using RNA-guided nucleic acid binding proteins

The present invention provides RNA-guided endonucleases, which are engineered for expression in eukaryotic cells or embryos, and methods of using the RNA-guided endonuclease for targeted genome modification in eukaryotic cells or embryos. Also provided are fusion proteins, wherein each fusion protein comprises a CRISPR/Cas-like protein or fragment thereof and an effector domain. The effector domain can be a cleavage domain, an epigenetic modification domain, a transcriptional activation domain, or a transcriptional repressor domain. Also provided are methods for using the fusion proteins to modify a chromosomal sequence or regulate expression of a chromosomal sequence.

CRISPR-based genome modification and regulation

The present invention provides methods and kits for editing specific chromosomal sequences in cells. In particular, targeting endonucleases and single-stranded nucleic acids are used to edit the chromosomal sequence.

Genome editing using targeting endonucleases and single-stranded nucleic acids

Disclosed herein are methods and compositions for genome editing of alocus, using fusion proteins comprising a zinc-finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins.

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A crab cage assembly includes a cage unit that has several one-way openings defined therethrough so that a crab can enter the cage but will not be able to exit that cage once inside. The one-way openings are formed by cantilever wires that extend into the cage. A signal system is activated when a captured crab pulls on a bait-like element located in the cage, and signals a crabber that a crab has been captured. The signal system includes either a bell or a special transmitter that emits a signal characteristic of the particular cage assembly so that a crabber working several cages will know which cage contains a crab.

Signalling crab cage

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St. Louis, MO, United States of America

Gregory D Davis