City of Hope

Sirna Therapeutics, Inc.

Gregory Copeland

Jacob Berlin

Hurik Muradyan

Kathleen Elison

Zhen Li

Daniel Zewge

Joseph D Armstrong

Provided herein are encoded split pool libraries useful, inter alia, for forming highly diverse and dense arrays for screening and detection of a variety of molecules.

Chemically encoded spatially addressed library screening platforms

This invention relates to a process for introducing two or more 2'-modifications into an RNA, wherein the RNA has a 2&#x2032;-O substituent containing an alkyl ester functional group on one or more ribose rings of a strand and a 2&#x2032;-O substituent containing an alkyne functional groups on one or more ribose rings on the same strand. The process comprises a) adding an amine compound to the RNA to form amidation reactions with the alkyl ester functional groups; b) dissolving the modified RNA from step (a) in a solvent to form a solution; and c) adding an organic azide and a copper or ruthenium catalyst to the solution obtained in step (b) to form 2&#x2032;-azide-alkyne cycloaddition reaction products with the alkyne functional groups.

Post-synthetic orthogonal amidation plus metal catalyzed azide-alkyne cycloaddition click chemistry on siRNA

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Claremont, CA, United States of America

Gregory Copeland