General Electric Company

Ge Healthcare Bio-sciences Corp.

Gregory Andrew Grossman

John Richard Nelson

Robert Scott Duthie

Anuradha Sekher

Christopher Michael Puleo

Paul Michael Smigelski, Jr

Erik Leeming Kvam

Victoria Eugenia Cotero

Kenneth Wayne Rigby

James Edward Rothman

Craig Patrick Galligan

Jason William Castle

John Donald Burczak

R Scott Duthie

Rohini Dhulipala

Methods of amplification, purification and detection of nucleic acid sequences especially RNA are described. One aspect of the method involves the hybridisation and subsequent ligation of a nucleic acid structure to the nucleic acid sequence desired to be manipulated. The methods require that the nucleic acid structure comprises a double stranded region and a single stranded region. The single stranded region is complementary to the RNA sequence of interest. The double stranded region may also contain additional functionalities which are then used subsequently in the method.

Ligation-based RNA amplification

A separation device, system and associated method are provided herein for separation of particulates form a base fluid. The separation device comprises a first microchannel comprising a fluid inlet and a mesofluidic collection chamber. The mesofluidic collection chamber has a first side and a second side, wherein the mesofluidic collection chamber is operatively coupled to the first microchannel on the first side, and wherein the mesofluidic collection chamber comprises a first fluid outlet at the second side, such that the fluid inlet, first microchannel, and first fluid outlet are in fluidic communication via the mesofluidic collection chamber.

Devices for separation of particulates, associated methods and systems

Provided herein are mutant endonuclease V enzymes that are capable of nicking an inosine-containing DNA sequence. Nucleic acid assays and agents that employ such mutant endonuclease V enzymes to introduce a nick into a target DNA including one or more inosine, and uses a DNA polymerase to generate amplicons of a target DNA are also described.

Mutant endonuclease V enzymes and applications thereof

Growing community of inventors

Halfmoon, NY, United States of America

Gregory Andrew Grossman