Technische Universitaet Ilmenau

Technische Universitat Ilmenau

Universitaetsklinikum Jena

Nanomics Technologies Gmbh

Universitatsklinikum Jena

Gregor Schlingloff

Andreas Schober

Frank Weise

Joerg Hampl

Uta Fernekorn

Hanns Wurziger

Michael Gebinoga

Holger Deppe

Alexander Gross

Justyna Borowiec

Dirk Tomandl

Beate Diefenbach

Jörg Hampl

Astrid Voigt

Angelika Mueller

James Beck

Sebastian Haefner

Sukhdeep Singh

Justyna Tobola

Angelika MÜLLER

Sebastian HÄFNER

Astrid Schaeffer

The invention relates to a microbioreactor assembly () having a plurality of microbioreactors. The microbioreactor assembly comprises a microtiter plate () having numerous wells () in a predefined grid arrangement, a closed bottom surface () and an open upper face. An insert unit () is also provided, which is arranged on the upper face of the microtiter plate () and has numerous inserts () in the same grid arrangement, each insert () engaging in a well (), and the well () being divided into at least two regions (). Finally, the microbioreactor assembly comprises an activation unit (), which is placed on the insert unit () and has numerous pumps (), each of which is connected to supply channels (), which allow the transport of fluid between the two regions ().

Microbioreactor assembly

The present invention relates firstly to a method for reproducing a stem cell niche of an organism. The invention further relates to a reproduction of a stem cell niche of an organism. According to the invention, an image of a tissue of an organism is generated, which tissue comprises at least one stem cell niche. The image is filtered in order to obtain a structural pattern of the imaged stem cell niche. In a further step, a lithographic mask is generated from the structural pattern. According to the invention, a starting material of a substrate is structured by means of indirect or direct application of the lithographic mask, whereby a structured substrate is obtained which represents the reproduction of the imaged stem cell niche of the organism. The reproduction can be characterised as biolithomorphic.

Reproduction of a stem cell niche of an organism and method for the generation thereof

A method is provided for producing a microstructured molded object that is intended for culturing of biological cells. According to this method, a plastically deformable first porous film is prepared, as well as a deformable second film and a deformable sacrificial film. The first, second and sacrificial film are placed in a stack. Next, the sacrificial film is subjected to pressure to press the stack into a mold. The mold has recesses, such that deformed regions in the form of cavities are produced in the sacrificial film, the first film and the second film, and undeformed regions remain. During the pressing of the film stack into the mold, the first film and the second film are joined to each other, so that they form a composite film. At least portions of the deformed regions of the second film are etched so that sections of the second film are chemically dissolved. In these sections of the second film, sections in the deformed regions of the first film are opened up, so that the pores in these sections are again free.

Method for production of a microstructured molded object

Microwells, which are open on both sides and are arranged two-dimensionally in a microwell matrix (), are hermetically closed by pressure on both sides using plugs () covered with a sealing sheet (). A layer of elastic material (), which is arranged between the plug () and the sealing sheet (), guarantees uniform pressure on all the microwells of a microwell matrix () which is loosely held by a frame structure (). Continuous peripheral elevations (), which protrude in both directions at right angles to the plane of the microwell matrix (), ensure that the deformable thin layer of elastic material () does not escape sideways between the plug () and the microwell matrix () during the pressing process. A plurality of microwell matrices () are held simultaneously by the frame structure () and are hermetically closed by a corresponding number of allocated plugs (), which are fastened to a base plate () and a cover plate (), respectively.

Device used in parallel microsynthesis

Growing community of inventors

Ilmenau, Germany

Gregor Schlingloff