Roche Molecular Systems, Inc.

Roche Diagnostics Gmbh

Roche Diagnostics Operations, Inc.

Boehringer Mannheim Gmbh

F. Hoffmann-la Roche Ag

Innogenetics N.v.

Gregor Sagner

Martin Gutekunst

Dieter Heindl

Hans-Peter Josel

Herbert Von Der Eltz

Rupert Herrmann

Rudi Rossau

Christoph Kessler

Thomas Emrich

Rudolf Seibl

Karim Tabiti

Richie Soong

Jutta Arden-Jacob

Klaus Muhlegger

Geert Jannes

Gudrun Schmitz-Agheguian

Astrid Reiser

Gerd Haberhausen

Heribert Maerz

J&ouml;rg Kleiber

Horst Domdey

Karl Heinz Drexhage

Christoph Mueller

Helmut Blum

Bruno Canard

Richard Robert De Haas

Rob Van Gijlswijk

Hans Tanke

Jorg Frantzeskos

Martin Moczko

Martin Horat

Rudolf Sebl

Jochen Bolte

Ingrid Bechler

Daniel De Vos

Joachim Bolte

Inga Boell

Anja Degen

The present invention provides a fluid transfer control method, the method based on measurements of intensities of dyes within the fluid to be transferred. In more detail, the present invention makes use of control dyes and quencher molecules for the fluid transfer controls.

Fluid transfer control for real-time PCR

The present invention concerns a method for the quantification of a target nucleic acid in a sample comprising the following steps: (i) determination of the amplification efficiency of the target nucleic acid under defined amplification conditions, (ii) amplification of the target nucleic acid contained in the sample under the same defined reaction conditions, (iii) measuring the amplification in real-time, (iv) quantification of the original amount of target nucleic acid in the sample by correction of the original amount derived from step (iii) with the aid of the determined amplification efficiency. The efficiency correction of PCR reactions according to the invention for the quantification of nucleic acids can be used for absolute quantification with the aid of an external or internal standard as well as for relative quantification compared to the expression of housekeeping genes.

Method for real-time quantification of nucleic acids

The invention is directed to a system for performing multi-color real time PCT, comprising a flexible real time PCT instrument and a specific composition or reaction mixture for performing multiplex PCR: In particular, the present invention is directed to a composition or reaction mixture which comprises at least 3, preferably 4-5 and most preferably exactly 4 pairs of FRET hybridization probes. Each pair of said hybridization probes consists of a FRET donor probe carrying a FRET donor moiety and a FRET acceptor probe carrying a FRET acceptor moiety having an emission maximum between 550 and 710 nm.

System for multi color real time PCR

Method for the efficiency-corrected real-time quantification of nucleic acids

The present invention concerns a method for determining the efficiency of the amplification of a target nucleic acid comprising the following steps: (i) preparation of a dilution series of the target nucleic acid, (ii) amplifying the target nucleic acid under defined reaction conditions, the amplification being measured in real-time (iii) setting a defined signal threshold value, (iv) determining the cycle number at which the signal threshold value is exceeded for various dilutions, (v) determining the amplification efficiency as a function of the amount of original target nucleic acid. The present invention also concerns a method for the quantification of a target nucleic acid in a sample in which the efficiency of the amplification reaction is determined in this manner and is taken into account in the quantification.

Method for determining the efficiency of nucleic acid amplifications

The present invention concerns a labelling reagent in which the label is bound via an amide bond and a linker to a residue of the molecule which is essentially characterized in that the N atom of the amide bond and the label are linked together directly by a covalent bond. In particular these are phosphoramidites or reactive supports suitable for nucleic acid synthesis. The invention also concerns processes for the production of such supports from suitable precursors.

Reagent for labelling nucleic acids

The present invention concerns a method and a device which enables an integrated amplification and detection of nucleic acids.

Integrated method and system for amplifying and for detecting nucleic acids

The invention concerns rhodamine derivatives of the general formulae

Rhodamine derivatives and the use thereof

The present invention refers to a process for the preparation of an activated metallo-porphyrin derivative and to novel activated metallo-porphyrin derivatives obtainable by said process. Further, the present invention refers to conjugates of metallo-porphyrins with molecules having at least one primary amino group, especially biomolecules. The conjugates are used in a procedure for the detection of a biological substance, especially in an immunoassay or a nucleic acid hybridization assay.

Use of metallo-porphyrin conjugates for the detection of biological

The invention is directed to the use of RNA or DNA polymerases having 3'-intrinsic editing activity (3'-IEA) in the presence or absence of a deactivating agent to remove a protecting group from the 3'-position of oligo- polyribo- or deoxyribonnucleotides. The use is connected with the incorporation of dNTPs into DNA templates in order to determine the concentration and/or sequence of said templates. In particular the use is concerned with an improved non gel-based sequencing method.

DNA polymerase having 3'-intrinsic editing activity

Simultaneous sequencing of nucleic acids

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Penzberg, Germany

Gregor Sagner