Pierce Biotechnology, Inc.

Dyomics Gmbh

Pierce Chemical Company

Greg Hermanson

Surbhi Desai

Peter T Czerney

Matthias S Wenzel

Frank G Lehmann

Boguslawa Dworecki

Marie Christine Nlend

Suk Hong

John Charles Rogers

Christopher Laurence Etienne

Wilhelm G Frank

Kay K Opperman

Barbara J Kaboord

Joel Louette

Jean-Samuel Schultz

Ryan Daniel Bomgarden

Bhavinkumar Patel

Patricia L Domen

Boguslawa R Dworecki

Compounds used as labels with properties comparable to known fluorescent compounds. The compounds can be conjugated to proteins and nucleic acids for biological imaging and analysis. Synthesis of the compounds, formation and use of the conjugated compounds, and specific non-limiting examples of each are provided.

Fluorescent compounds

Compounds useful as labels with properties comparable to known fluorescent compounds. The compounds are conjugated to proteins and nucleic acids for biological imaging and analysis. Synthesis of the compounds, formation and use of the conjugated compounds, and specific non-limiting examples of each are provided.

Benzocyanine compounds

Cyanine compounds

Compounds used as labels with properties comparable to known fluorescent compounds. The compounds are conjugated to proteins and nucleic acids for biological imaging and analysis. Synthesis of the compounds, formation and use of the conjugated compounds, and specific non-limiting examples of each are provided.

Benzopyrylium compounds

A phosphine derivative of DyLight dyes modified with ethylene glycol or (poly)ethylene glycol groups. In one embodiment, the compounds are useful in chemoselective ligation reactions.

Phosphine derivatives of fluorescent compounds

A method for absolute protein or peptide quantitation by mass spectroscopy. A sample containing a protein or peptide of interest is prepared for mass spectroscopy analysis. The sample is subjected to mass spectroscopy analysis at low resolution whereby a single additive mass spectroscopy peak is obtained, then is subjected to high resolution mass spectroscopy analysis whereby a plurality of mass spectroscopy peaks are obtained. The intensity of each of the plurality of mass spectroscopy peaks is quantitated either by comparison to an internal standard set, or by using a standard curve generated for each isotopologue set. Quantitation using a standard curve enhances quantitation across a dynamic range of analyte.

Absolute quantitation of proteins and protein modifications by mass spectrometry with multiplexed internal standards

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Loves Park, IL, United States of America

Greg Hermanson