Ortho-Clinical Diagnostics, Inc

Orasure Technologies, Inc.

Graham Yearwood

Banumathi Sankaran

Philip C Hosimer

Sheryl S Sullivan

Darrell C Haynes

Michael Reed

Disclosed herein are assay methods, lateral flow assay test strips, and devices for improved analyte detection. Analyte binding to target is performed both in solution phase and with a target immobilized on a surface, resulting in improved analyte detection.

Assay methods for improved analyte detection

An immunoassay reagent is provided which comprises an analyte binding agent in a diluent, and a glycosaminoglycan in an amount sufficient to reduce non-specific binding in an assay of a sample for the analyte. Provided is such an immunoassay reagent in which the analyte is troponin I, the analyte binding agent is a biotinylated anti-troponin I antibody, and the glycosaminoglycan is chondroitin sulfate. A sample composition is also provided which comprises a sample to be assayed for the presence of an analyte, an analyte binding agent, and a glycosaminoglycan other than heparin. Further provided is a method of detecting an analyte in a sample, in which non-specific binding is reduced in the method using a glycosaminoglycan.

Use of glycosaminoglycans to reduce non-specific binding in immunoassays

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Bethlehem, PA, United States of America

Graham Yearwood