Exact Sciences Corporation

Exact Sciences Development Company, LLC

Mayo Foundation for Medical Education and Research

Other

Graham P Lidgard

Hatim T Allawi

Michael J Domanico

Hongzhi Zou

William Russell Taylor

David Alan Ahlquist

Douglas W Mahoney

William G Weisburg

Rebecca Oldham-Haltom

Keith Kopitzke

James P Light, Ii

Michael W Kaiser

Janelle J Bruinsma

Hemanth D Shenoi

John B Kisiel

Tracy C Yab

Abram Michael Vaccaro

John Zeis

Brian Aizenstein

Keith D Fourrier

Scott E Castanon

Tamara J Sander

Zubin Gagrat

Maria Giakoumopoulos

Ilse A Tyler

Oliver Hunt

Melissa M Gray

Tobias Charles Zutz

Daniel J Simpson

Kathleen S Harings

Jacquelyn T Hennek

Keith Yaeger

Gracie Shea

Chateen Krueger

William R Taylor

David A Ahlquist

Martin Joseph Bouliane

Joseph E Schiestle

Mike Domanico

James P Light

Keith Fourrier

Scott Castanon

Ii Light James P

Hatim Allawi

James P Ii Light

Ii James P Light

Provided herein is technology for breast cancer screening and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of breast cancer.

Detecting breast cancer

Provided herein is technology relating to isolating nucleic acids. In particular, the technology relates to methods and kits for extracting nucleic acids from problematic samples such as stool.

Isolation of nucleic acids

Provided herein is technology relating to performing methylation assays. In particular, the technology relates to internal controls for methylation assays.

Compositions and methods for performing methylation detection assays

A cleavage-based real-time PCR assay method is provided. In general terms, the assay method includes subjecting a reaction mixture comprising a) PCR reagents for amplifying a nucleic acid target, and b) flap cleavage reagents for performing a flap cleavage assay on the amplified nucleic acid target to two sets of thermocycling conditions. No additional reagents are added to the reaction between said first and second sets of cycles and, in each cycle of the second set of cycles, cleavage of a flap probe is measured.

Real time cleavage assay

Provided herein is technology relating to the amplification-based detection of nucleic acids and particularly, but not exclusively, to methods and compositions for minimizing variability in the activity between different samples or manufacturing lots of DNA polymerases, such as Taq DNA polymerase.

Normalization of polymerase activity

Provided herein is technology for esophageal disorder screening and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of esophageal disorders (e.g., Barrett's esophagus, Barrett's esophageal dysplasia, etc.). In addition, the technology provides methods, compositions and related uses for distinguishing between Barrett's esophagus and Barrett's esophageal dysplasia, and between Barrett's esophageal low-grade dysplasia, Barrett's esophageal high-grade dysplasia, and esophageal adenocarcinoma within samples obtained through endoscopic brushing or nonendoscopic whole esophageal brushing or swabbing using a tethered device (e.g. such as a capsule sponge, balloon, or other device).

Detecting esophageal disorders

A method for detecting a methylated genomic locus is provided. In certain embodiments, the method comprises: a) treating a nucleic acid sample that contains both unmethylated and methylated copies of a genomic locus with an agent that modifies cytosine to uracil to produce a treated nucleic acid; b) amplifying a product from the treated nucleic acid using a first primer and a second primer, wherein the first primer hybridizes to a site in the locus that contain methylcytosines and the amplifying preferentially amplifies the methylated copies of the genomic locus, to produce an amplified sample; and c) detecting the presence of amplified methylated copies of the genomic locus in the amplified sample using a flap assay that employs an invasive oligonucleotide having a 3' terminal G or C nucleotide that corresponds to a site of methylation in the genomic locus.

Methylation assay

Provided herein is technology relating to the amplification-based detection of bisulfite-treated DNAs and particularly, but not exclusively, to methods and compositions for multiplex amplification of low-level sample DNA prior to further characterization of the sample DNA. The technology further provides methods for isolating DNA from blood or blood product samples, e.g., plasma samples.

Multiplex amplification detection assay II

Provided herein is technology for lung neoplasia screening and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of lung cancer.

Detection of lung neoplasia by amplification of RNA sequences

Provided herein is reagent mixture comprising multiplexed amplification reagents and flap assay reagents for detecting, in a single reaction, mutant copies of the KRAS gene that contain any of the 34A, 34C, 34T, 35A, 35C, 35T or 38A point mutations. Methods that employ the reagent mix and kits for performing the same are also provided.

Multiplexed KRAS mutation detection assay

Provided herein is technology for neoplasia screening, and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of cancer, in particular, colorectal cancer.

Detection of colon neoplasia by analysis of methylated DNA

Provided herein is technology relating to collecting and preparing samples. For example, the technology relates particularly, but not exclusively, to devices, systems, and kits that allow a subject to collect and prepare a sample for analysis.

Sample collection device

A stool resuspension solution comprising a hemoglobin stabilization reagent is provided. In some embodiments, the hemoglobin stabilization reagent may be an osmolyte, a polyvalent cation, a sugar or polysaccharide and, optionally, a polyvalent cation, a protoporphyrin, or an HRP stabilization component and, optionally, a polyvalent cation. A method of stabilizing hemoglobin in a stool sample in the solution is also provided, as well as a sample collection device containing the solution.

Method for stabilizing hemoglobin and reagents for performing the same

Provided herein is technology relating to detecting neoplasia and particularly, but not exclusively, to methods, compositions, and related uses for detecting premalignant and malignant neoplasms such as colorectal cancer.

Detecting colorectal neoplasm

Growing community of inventors

Madison, WI, United States of America

Graham P Lidgard