Medisense, Inc.

Other

Genetics International, Inc.

Graham Davis

Hugh A Hill

Irving J Higgins

James Michael McCann

Hugh Allen Hill

A sensor system that detects a current representative of a compound in a liquid mixture features a two-electrode strip adapted for releasable attachment to signal readout circuitry. The strip comprises an elongated support (preferably flat) adapted for releasable attachment to the readout circuitry; a first conductor and a second conductor each extend along the support and comprise means for connection to the circuitry. An active electrode, positioned to contact the liquid mixture and the first conductor, comprises a single layer deposit of an enzyme capable of catalyzing a reaction involving the compound, a conductive material and an electron mediator, capable of transferring electrons between the enzyme-catalyzed reaction and the first conductor. A reference electrode is positioned to contact the mixture and the second conductor. The system includes circuitry adapted to provide an electrical signal representative of the current. The two-electrode strip is manufactured, e.g., by screen printing an admixture of an enzyme, a conductive material, and a mediator as a single layer onto the substrate.

Strip electrode with screen printing

A sensor system that detects a current representative of a compound in a liquid mixture features a two-electrode strip adapted for releasable attachment to signal readout circuitry. The strip comprises an elongated support (preferably flat) adapted for releasable attachment to the readout circuitry; a first conductor and a second conductor each extend along the support and comprise means for connection to the circuitry. An active electrode, positioned to contact the liquid mixture and the first conductor, comprises a single layer deposit of an enzyme capable of catalyzing a reaction involving the compound, a conductive material, and an electron mediator, capable of transferring electrons between the enzyme-catalyzed reaction and the first conductor. A reference electrode is positioned to contact the mixture and the second conductor. The system includes circuitry adapted to provide an electrical signal representative of the current. The two-electrode strip is manufactured, e.g., by screen printing, an admixture of an enzyme a conductive material, and a mediator as a single layer onto the substrate.

A sensor system that detects a current representative of a compound in a liquid mixture features a two-electrode strip adapted for releasable attachment to signal readout circuitry. The strip comprises an elongated support (preferably flat) adapted for releasable attachment to the readout circuitry; a first conductor and a second conductor each extend along the support and comprise means for connection to the circuitry. An active electrode, positioned to contact the liquid mixture and the first conductor, comprises a deposit of an enzyme capable of catalyzing a reaction involving the compound and (preferably) an electron mediator, capable of transferring electrons between the enzyme-catalyzed reaction and the first conductor. A reference electrode is positioned to contact the mixture and the second conductor. The system includes circuitry adapted to provide an electrical signal representative of the current. The two-electrode strip is manufactured, e.g., by screen printing the enzyme (and preferably the mediator) onto the substrate.

A sensor system that detects a current representative of a compound in a liquid mixture features a two-electrode strip adapted for releasable attachment to signal readout circuitry. The strip comprises an elongated support (preferably flat) adapted for releasable attachment to the readout circuitry; a first conductor and a second conductor each extend along the support and comprise means for connection to the circuitry. An active electrode, positioned to contact the liquid mixture and the first conductor, comprises a deposit of an enzyme capable of catalyzing a reaction involving the compound and (preferably) an electron mediator, capable of transferring electrons between the enzyme-catalyzed reaction and the first conductor. A reference electrode is positioned to contact the mixture and the second conductor. The system includes circuitry adapted to provide an electrical signal representative of the current. The two-electrode strip is manufactured, e.g., by screen printing an admixture of enzyme, a conductive material, and a mediator as a single layer onto the substrate.

Strip electrode including screen printing of a single layer

This specification discloses methods of detection or measurement of an enzyme or of its specific substrate, and sensors used in such methods. The present invention is concerned with a multi-enzyme system, and the specification discloses, as one aspect of the invention a method of assay in which an electrode poised at a suitable potential is contacted with a system comprising a first enzyme, a cofactor linked with said enzyme and a mediator compound which transfers charge to the electrode from the first enzyme when its electrical state is changed by reaction of cofactor material. The cofactor may be NAD, NADP (both collectively referred to herein as NAD(P)), cAMP, ATP, GTP, TTP, or CTP. The specification particularly illustrates a method of assay in which an electrode (1) poised at a suitable potential is contacted with a system comprising a first enzyme E.sub.1 a nicotinamide adenine dinucleotide compound N linked with said enzyme E.sub.1 and a mediator compound F which transfers charge to the electrodes from the first enzyme when its electrical state is changed by a NAD(P) NAD(P)H reaction. The NAD compound may act as a 'bridge' between the said enzyme/mediator system and further NAD utilizing enzyme E2.

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Plainsborough, NJ, United States of America

Graham Davis