Chiron Corporation

Graeme Ian Bell

Carl-Henrik Heldin

Bengt Westermark

James Scott

Timothy J Knott

Christer Betsholtz

Guy T Mullenbach

Robert A Hallewell

Pablo D Valenzuela

Lawrence S Cousens

James P Merryweather

Leslie B Rall

DNA encoding two forms of PDGF A-chain polypeptide, the construction of expression vectors for expressing such DNA in yeast and mammalian cells, and the expression of such DNA in yeast and mammalian cells to produce active PDGF A-chain homodimer and active PDGF A-chain/B-chain heterodimer are disclosed.

Recombinant PDGF A-chain homodimers and methods of use

Manganese superoxide dismutase cloning and expression in microoganisms

Manganese superoxide dismutase cloning and expression in microorganisms

Recombinant DNA encoding PDGF A-chain polypeptides

Polynucleotide sequences which encode for human prepro insulin-like growth factors are provided. Such sequences are obtained from the human genome, typically by screening a cDNA library obtained from human liver cells. The polynucleotide sequences may be used for cloning and expression of insulin-like growth factors in suitable hosts, as well as for the production of DNA and RNA which may be used as hybridization probes. E. coli strains HB101(phigf1) and HB101(phigf2) were deposited at the ATCC on Jun. 8, 1984, and granted accession nos. 39729 and 39730, respectively.

Prepro insulin-like growth factors I and II

Recombinant DNA encoding PDGF A-chain polypeptide and expression vectors

DNA sequences and methods of obtaining DNA sequences which include a sequence encoding for mammalian epidermal growth factor are provided. The DNA sequences may be used in cloning and expression vectors for production of DNA and RNA and for producing polypeptides including mammalian epidermal growth factor. E. coli strains HB 101 (pmegf1) and HB 101 (pmegf10b), and bacteriophage .lambda.hEGF34 were deposited at the A.T.C.C. on May 10, 1983 and given Accession Nos. 39357, 39358 and 40070, respectively.

Hybrid DNA synthesis of epidermal growth factor

The adenovirus major late promoter is employed as a promoter for expression in a yeast host. Constructions are provided for expression in yeast with the adenovirus major late promoter and a coding segment.

Adenovirus promoter system

Growing community of inventors

San Francisco, CA, United States of America

Graeme Ian Bell