Nexttec Gmbh

Apogene Gmbh & Co. Kg

Apogene Biotechnologie Gmbh & Co. Kg

Bavarian Nordic Research Institute A/s

Gottfried Brem

Robert-Matthias Leiser

Lutz Plobner

Hamlet Balayan

Dieter Klein

Vitali Pavlovich Zubov

Leonti E Tkachenko

Ralf Steinborn

Dimitri Valerjewich Kapoustine

Mathias M&uuml;ller

Thomas Czerny

The present invention relates to a sorbent material for separation and purification of biopolymers, particularly nucleic acids, having a solid support substantially modified with a copolymer coating comprising aromatic monomers and crosslinking compounds and unsaturated esters or ethers preferably attached to the support via a vinylchlorsilane. The use of these materials for separation of nucleic acids, particularly a one-step isolation of DNA from lysates of different biological sources, is an object of the invention as well as a chromatographic column or cartridge at least partially filled with the sorbent material of the invention, a membrane-like device comprising the sorbent material of the invention, and a kit comprising the sorbent material of the invention in bulk or packed in chromatographic devices as well as other devices necessary for performing sample preparations.

Process for manufacturing a composite sorbent material for chromatographical separation of biopolymers

The present invention relates to a method for recombinant manufacturing of substances, wherein cells are transformed suing a nucleotide sequence coding for the substance, the transformed cells are subjected to a cloning process, and the cells obtained in this fashion are introduced into a host organism. The present invention relates in particular to the use of the method in the production of recombinant proteins, cells and tissues. According to a further aspect, the invention relates to a method, wherein the cells of an individual are isolated, said sells are introduced into an immunoincompetent animal for further growth and the cells, tissue and/or organs cultivated in the animal are again isolated and introduced into an individual.

Somatic cloning gene transfer for the production of recombinant proteins, cells and organs

A composite polymer-coated sorbent with a bidisperse or oligodisperse distribution of pore sizes and having an at least partial coating on its surface, which coating comprises essentially polyanilines or derivatives of polyanilines and use thereof for the simultaneous separation and desalting of bio-macromolecules.

Composite polymer-coated sorbent with a bidisperse pore size distribution for the simultaneous separation and desalting of biopolymers

The invention relates to a simple and rapid method for identifying mutations in a gene of interest indicating an alteration in the reading frame of the proteins derived therefrom. The inventive method more particularly relates to the use of said method for scanning the animal population.

Method for identifying reading frame mutations

The present invention provides a method of quantification of nucleic acid sequences with minimal alterations conducting a real time PCR quantification assay with allele-specific primers. Further, the present invention provides the method for quantifying allelic differences of bovine mitochondrial DNA sequences and allele-specific primers to be used in said method.

Quantification by inhibition of amplication

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Hilgertshausen, Germany

Gottfried Brem