Leland Stanford Junior University

Cetus Corporation

Berlex Laboratories, Inc.

Gordon M Ringold

Michael A Innis

Francis P McCormick

James W Larrick

David F Mark

Leo S Lin

Frank M Torti

.beta.-interferon is produced in Chinese hamster ovary cells (CHO) in high amounts.

Human interferon-.beta. (IFN-.beta.) produced in Chinese hamster ovary

DNA constructs are prepared which operably link human interferon genes, selective, eukaryotic marker genes, and promoter and expression control sequences for the expression of human interferon in Chinese hamster ovary (CHO) cells or progeny thereof. The human recombinant interferon so produced contains glycans which are a subset of the population of glycans which are contained in the native counterpart, and may be used in therapeutic formulations. The CHO cells yield high levels of human interferon with no detectable amounts of host, IFN, either constitutive or inductive.

Expression of interferon genes in Chinese hamster ovary cells

DNA constructs are prepared which operably link human interferon genes, selective, eukaryotic marker genes, and promoter and expression control sequences for the expression of human interferon in Chinese hamster ovary (CHO) cells or progeny thereof. The human recombinant interferon so produced contains glycans which are a subset of the population of glycans which are contained in the native counterpart, and may be used in therapeutic formulations. The CHO cells yield high levels of human interferon with no detectable amounts of host IFN, either constitutive or inductive.

A method for controlling weight by suppressing the normal metabolism of adipose tissue is disclosed. Administration of tumor necrosis factor (TNF) or a pharmaceutical composition containing it results in suppression of anabolism of adipose cells.

Use of tumor necrosis factor as a weight regulator

Method and compositions are provided for gene amplification. A DNA unit is prepared capable of replication in a eukaryotic host having tandem genes, where the first gene is capable of complementing an auxotrophic host. The auxotrophic host is transformed with the DNA unit under selective conditions requiring enhanced expression of the first gene. Hosts are then selected for amplification of the first and second genes.

Growing community of inventors

Palo Alto, CA, United States of America

Gordon M Ringold