Columbia University

Massachusetts Institute of Technology

Tufts College

Other

The General Hospital Corporation

Gordana Vunjak-Novakovic

Keith Yeager

David L Kaplan

John O'Neill

Sarindr Bhumiratana

Matthew D Bacchetta

Lorenz Meinel

Warren L Grayson

Kacey Ronaldson

Robert Samuel Langer

Lisa E Freed

Rina Nazarov

Timothy Chen

Brandon Guenthart

Stephen Ma

Donald O Freytes

Ivan Martin

Nina Tandon

Michael L Lovett

Rina Sears

Scott Chicotka

Kenmond Fung

Christopher M Cannizzaro

Jinho Kim

Gregory H Altman

Gopal Singh

Xianyan Wang

Scott A Kanner

Charu P Vepari

Milica Radisic

Hyoungshin Park

Henning Madry

Leo Qun Wan

Stephen B Trippel

Joshua Sonett

Hesam Parsa

Aranzazu Villasante

Qun Wan

Elisa Cimetta

Amandine Godier-Furnémont

George Eng

Robert Maidhof

Described are systems, methods, and devices relating to normothermic extracorporeal support of an organ, tissue, or bioengineered graft comprising cross-circulation (XC) perfusion for prolonged periods (days to weeks) via an XC perfusion circuit in connection with an extracorporeal host (e.g., animal, patient, organ transplant recipient) are disclosed. The XC perfusion circuit comprises auto-regulation of blood flow based on the trans-organ blood pressure difference between arterial and venous pressure. Recipient support enabled 36 h of normothermic perfusion that maintained healthy lungs with no significant changes in physiologic parameters and allowed for the recovery of injured lungs. Extended support enabled multiscale therapeutic interventions in all extracorporeal lungs. Lungs exceeded transplantation criteria.

Cross-circulation platform for recovery, regeneration, and maintenance of extracorporeal organs

A cardiac organoid containing 3-D matter of adult human heart tissue.

Engineered adult-like human heart tissue

Medical devices having engineered mechanically functional cartilage from adult human mesenchymal stem cells and method for making same.

Engineering mechanically functional human cartilage and method of making same

The present invention relates to a computer-vision based autonomous steerable catheter device and methods of using the same for targeted delivery of a liquid microvolume into the interior of a lung.

Method and apparatus for computer-vision guided targeted delivery of small liquid volumes into selected lung regions

The present disclosure relates to a pneumatic microfluidic platform for high-throughput studies of cardiac hypertrophy that enables repetitive (hundreds of thousands of times) and robust (over several weeks) manipulation of cardiac &#x3bc;tissues. The platform is reusable for stable and reproducible mechanical stimulation of cardiac &#x3bc;tissues (each containing only 500 cells). Heterotypic and homotypic &#x3bc;tissues produced in the device were pneumatically loaded in a range of regimes, with real-time on-chip analysis of tissue phenotypes. Concentrated loading of the three-dimensional cardiac tissue faithfully recapitulated the pathology of volume overload seen in native heart tissue. Sustained volume overload of &#x3bc;tissues was sufficient to induce pathological cardiac remodeling associated with upregulation of the fetal gene program, in a dose-dependent manner.

Smart micro bioreactor platform for high throughput mechanical stimulation of cardiac microtissue

An anatomically-shaped, human bone graft may be cultivated ex vivo using a bioreactor capable of perfusing large complex porous scaffolds. Scaffolds derived from image-based modeling of a target are seeded with human mesenchymal stem cells and cultivated. A bioreactor configured to house complex three-dimensional scaffold geometries provides controlled flow for perfusion of the cells. Dense uniform cellular growth can be attained throughout the entire scaffold as a result of the medium perfusion. In an embodiment, the bioreactor has a mold into which perfusion medium is pumped under pressure and multiple ports through which the medium exits the mold.

Method of making a personalized bone graft

Disclosed are a biosealant system and method for treatment of a pulmonary air leak comprising applying the biosealant system to the locus of the air leak.

Cell-seeded porous lung hydrogel sealant

A bioreactor is provided that permits engineering of multiple different tissues. The bioreactor has a series of flow paths that permit application of tissue-specific media while simultaneously innervating the various different tissues with a common media. The flow paths for the various medias are designed to prevent mixing of the various media as they simultaneously innervate the tissue.

Bioreactor system for engineering tissues

The present invention provides processes for producing porous silk fibroin scaffold material. The porous silk fibroin scaffold can be used for tissue engineering. The porosity of the silk fibroin scaffolds described herein can be adjusted as to mimic the gradient of densities found in natural tissue. Accordingly, methods for engineering of 3-dimensional tissue, e.g. bone and cartilage, using the silk fibroin scaffold material are also provided.

Silk fibroin materials and use thereof

A perfusion bioreactor chamber for engineering a broad spectrum of tissues. The bioreactor allows controlled distribution of fluid through or around scaffolding materials of various shapes, structures and topologies during prolonged periods of cultivation.

Perfusion bioreactor with tissue flow control and live imaging compatibility

A 3D decellularized bone scaffold seeded with cancer cells, such as prostate cancer cells or Ewing's sarcoma is provided. The three-dimensional includes Ewing's sarcoma (ES) tumor cells; and an engineered human bone scaffold. The engineered human bone scaffold further includes osteoblasts that secrete substance of the human bone, and osteoclasts that absorb bone tissue during growth and healing. The engineered human bone scaffold includes the tissue engineered three-dimensional model which recapitulates the osteolytic process. The engineered human bone scaffold is engineered by co-culturing of osteoblasts and osteoclasts. The osteoblast is produced by cell differentiation process from mesenchymal stem cells. The osteoclast is produced by cell differentiation from human monocytes, wherein the human monocytes are isolated from buffy coats. The scaffold can be used with cancer cell lines to identify therapeutic targets to slow, stop, and reverse tumor growth and progression as well as to predict the efficacy of potential therapeutics.

Tissue-engineered three-dimensional model for tumor analysis

Tissue engineering system for making personalized bone graft

Growing community of inventors

New York, NY, United States of America

Gordana Vunjak-Novakovic