Pharmacia Ab

Pharmacia Lkb Biotechnology Ab

Goran Kronvall

Lars Bjorck

Gunnar Lindahl

William H Kastern

A hybrid DNA molecule is disclosed by this invention capable of cellular expression comprising the DNA sequence for protein G. Said DNA sequence has substantially the same IgC binding properties as naturally occurring protein G. The molecule may be comprised of DNA sequences coding for fragments of protein G. Moreover, said DNA sequence may code for both fragments of protein G and protein G. The DNA sequence can be isolated from streptococcal DNA.

Protein G and/or fragments thereof

A process is disclosed for recovering a cell wall protein, particularly a Fc-receptor, from streptococcal bacteria. In the process, streptococcal bacteria are treated with at least one proteolytic enzyme to solubilize the cell wall protein. A process is also disclosed for recovering Fc-receptor type III (protein G) having selective IgG-binding capability (i.e., no albumin-binding capability) from streptococcal bacteria by pretreating these bacteria with enzymes prior to said enzymatic solubilization.

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Lund, Sweden

Goran Kronvall