Danisco US Inc.

The Goodyear & Tire Rubber Company

Butamax Advanced Biofuels LLC

Genencor International, Inc.

E.i. Dupont De Nemours and Company

Gopal K Chotani

Joseph Charles McAuliffe

Karl John Sanford

Gregory Marshall Whited

Marguerite A Cervin

Caroline M Peres

Richard La Duca

Fernando Valle

Jayarama K Shetty

Frank James Feher

Aaron Scott Puhala

Andrei Miasnikov

Anthony R Calabria

Michael C Miller

Bruce A Strohm

Michael Jay Pepsin

Timothy A Sabo

Nigel S Dunn-Coleman

Benjamin S Bower

Suzanne E Lantz

Toby M Baldwin

Erin L Spring

Vivek Sharma

Carol Marie Fioresi

Gang Duan

Oreste J Lantero, Jr

Ilana S Aldor

Daniel P O'Keefe

Derek H Wells

Mark J Nelson

Jean-Francois Tomb

Alex Toftgaard Nielsen

Sung Ho Lee

Michael Dauner

Jahnavi Chandra Prasad

Ritu Bhalla

Ying Qian

Jacob Andrew Latone

Hongxian Xu

Manoj N Kumar

Walter Weyler

Oreste J Lantero

William Albert Cuevas

Craig E Pilgrim

Jeff P Pucci

Dmitrii V Vaviline

Paula Johanna Maria Teunissen

Rachel E Muir

Robin Fong

Michael W Schelle

Jaclyn Diana Demartini

Floor K Kooy

Collette M Blake

Douglas Ko

Matt Reboli

Pauline Johanna Maria Teunissen

Martien H Bergsma

Brian Kirshner

Evan Sims

Erin L Webster

Kenneth F Herfert

Janine Reimann

Anthony Rudolf Calabria

Joseph C Mcauliffe

Timothy Alan Sabo

Erin Lynne Webster

William A Cuevas

Manoj Kumar

Michael Charles Miller

The present invention relates to a dry grind ethanol process in which post-distillation backset is subjected to aerobic fermentation to remove components that are inhibitory to an ethanolagen, such as yeast, allowing the utilization of an increased amount of post-distillation backset during the initial preparation of starch-containing substrates for the dry grind ethanol process. Aerobic fermentation of the post-distillation backset allows substantially higher backset recycle, resulting in fresh water savings and increased sustainability.

Method for increasing backset recycle in dry grind alcohol production

The invention features methods for producing isoprene from cultured cells wherein the cells in the stationary phase. The invention also provides compositions that include these cultured cells and/or increased amount of isoprene. The invention also provides for systems that include a non-flammable concentration of isoprene in the gas phase. Additionally, the invention provides isoprene compositions, such as compositions with increased amount of isoprene or increased purity.

Compositions and methods for producing isoprene free of c5 hydrocarbons under decoupling conditions and/or safe operating ranges

The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.

Compositions and methods for producing isoprene

The present disclosure provides methods, compositions and apparatuses for increasing gas transfer in fermentation processes.

Use of hydrophobins to increase gas transfer in aerobic fermentation processes

Provided herein are glycerol-3-phosphate dehydrogenase (GPD) enzymes with increased Kfor NADH and GPD enzymes with substantially the same affinity for NADH and NADPH and/or are feedback inhibited by glycerol-3-phosphate. Also provided herein are recombinant microorganisms comprising a heterologous gene encoding GPD and a deletion or disruption in an endogenous gene encoding GPD. Also provided are recombinant microorganisms comprising a heterologous gene encoding GPD and a butanol biosynthetic pathway. Further provided are methods of producing butanol comprising providing the recombinant microorganisms described herein and contacting the recombinant microorganism with at least one fermentable carbon substrate under conditions wherein butanol is produced.

Glycerol 3-phosphate dehydrogenase for butanol production

Compositions and methods for producing isoprene free of C5 hydrocarbons under decoupling conditions and/or safe operating ranges

The invention features methods producing isoprene and a co-product, such as ethanol, 1,3-propanediol, or hydrogen from cultured cells. The invention also provides compositions that include these cultured cells. The invention provides compositions comprising isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen. Additionally, the invention provides methods of co-producing isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen by culturing cells under conditions suitable for co-production of isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen.

Methods of producing isoprene and a co-product

Glycerol 3- phosphate dehydrogenase for butanol production

The present invention relates to filamentous fungal host cells and particularlyhost cells useful for the production of heterologous granular starch hydrolyzing enzymes having glucoamylase activity (GSHE). Further the invention relates to a method for producing a glucose syrup comprising contacting a granular starch slurry obtained from a granular starch substrate simultaneously with an alpha amylase and a GSHE at a temperature equal to or below the gelatinization temperature of the granular starch to obtain a composition of a glucose syrup.

Expression of granular starch hydrolyzing enzymes in trichoderma and process for producing glucose from granular starch sustrates

Provided herein are compositions and methods related to the direct conversion of the starch in a ground or fractionated grain into a fermentable sugar feedstock capable of serving as a carbon source for the industrial production of one or more products by a fermenting organism, such as isoprene, isoprenoid precursor molecules, and/or isoprenoids. Such conversions may be performed at temperatures at or below the initial gelatinization temperature of the starch present in the grain and may utilize one or more isolatable endogenous enzymes present in certain unrefined grains.

Direct starch to fermentable sugar as feedstock for the production of isoprene, isoprenoid precursor molecules, and/or isoprenoids

It has been found that certain cells in culture can convert more than about 0.002 percent of the carbon available in the cell culture medium into isoprene. These cells have a heterologous nucleic acid that (i) encodes an isoprene synthase polypeptide and (ii) is operably linked to a promoter. The isoprene produced in such a cultured medium can then be recovered and polymerized into synthetic rubbers and other useful polymeric materials. The synthetic isoprene containing polymers of this invention offer the benefit of being verifiable as to being derived from non-petrochemical based resources. They can also be analytically distinguished from rubbers that come from natural sources. The present invention more specifically discloses a polyisoprene polymer which is comprised of repeat units that are derived from isoprene monomer, wherein the polyisoprene polymer has &#x3b4;C value of greater than &#x2212;22%.

Polymers of isoprene from renewable resources

The invention features methods for producing isoprene from cultured cells wherein the cells in the stationary phase. The invention also provides compositions that include these cultured cells and/or increased amount of isoprene. The invention also provides for systems that include a nonflammable concentration of isoprene in the gas phase. Additionally, the invention provides isoprene compositions, such as compositions with increased amount of isoprene or increased purity.

The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells. The invention provides isoprene compositions, such as compositions with increased amount of isoprene or increased purity. Additionally, the invention provides methods of producing isoprene by culturing cells under conditions suitable for isoprene production while maintaining cell viability and/or metabolic activity.

Systems using cell culture for production of isoprene

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Cupertino, CA, United States of America

Gopal K Chotani