Seoul National University

Lart Bio Co., Ltd

Snu Rdb Foundation

Hanwha Advanced Materials Corporation

Goo Jang

Byeong Chun Lee

Jong Ik Hwang

Curie Ahn

Jae Seok Yang

Bum Rae Cho

Jung Taek Kang

Dae Kee Kwon

Ok Jae Koo

Min Kyu Kim

Hye Jin Kim

Sung Keun Kang

Dae Yong Kim

Jung Park

Hyun Ju Oh

Sol Park

Fibrianto Yuda

M Shamim Hossein

Joung Joo Kim

So Gun Hong

Byoung Gon Moon

Wonyou Lee

Dong Hyeok Kwon

Gyeongmin Gim

Gyeong Min Gim

Soo Young Yum

Won You Lee

The present specification relates to a transgenic animal and a transgenic embryo producing components of an engineered nuclease. According to the disclosure of the present specification, the transgenic animal (or embryo) producing components of an engineered nuclease is a transgenic animal (or embryo) which includes a first cell having a genome including a first toolbox; and a second cell having a genome including a second toolbox, wherein the first toolbox and the second toolbox include at least one of a polynucleotide encoding an RNA-guided endonuclease and a polynucleotide encoding a guide nucleic acid that is able to specifically bind to a target site, respectively, wherein the first toolbox is present in a first locus of the genome of the first cell; the second toolbox is present in a second locus of the genome of the second cell; and the first locus is different from the second locus.

Transgenic animals and transgenic embryos producing an engineered nuclease

Provided is a method for more efficiently sorting out genetically modified cells. Specifically provided are a method for selecting a cell including a modified gene on a target locus in a genome, a method for producing a cell including a modified gene on a target locus in a genome, and an animal including a modified gene on a target locus in a genome, and a kit for selecting an animal including a modified gene on a target locus in a genome and cells including a modified gene on a target locus in a genome.

Method and composition for sorting out of cell comprising a modified gene

The present invention relates to a transgenic pig that expresses sTNFR1-Fc, wherein a gene encoding sTNFR1-Fc, which is a fusion protein of the extracellular domain of human soluble tumor necrosis factor receptor (sTNFR1) and an immunoglobulin Fc region, is introduced; a method for preparing the same; an organ isolated from the transgenic pig; a somatic donor cell line inserted with sTNFR1-Fc gene; a method for preparing a blood sample comprising sTNFR1-Fc; and a method for preparing human sTNFR1-Fc from the blood sample of the transgenic pig. As the transgenic pig can suppress immune response and inflammatory response by secreting an inhibitory substance that suppresses the activity of TNF-&#x3b1; in blood, it can be effectively used for xenograft. Furthermore, since the transgenic pig has a blood type O, it can be transplanted for suppressing inflammatory response, regardless of a blood type of recipient.

Transgenic pig expressing sTNFR1-Fc genes and the uses thereof

The present invention relates to a method for producing a transgenic pig in which immune rejection response is inhibited, and in which human HO-1 genes and TNFR1-Fe fusion genes are simultaneously expressed. The present invention also relates to a transgenic pig for organ transplantation, which is produced by the method, and in which immune rejection response is inhibited. The present invention also relates to a somatic-cell-donating cell strain for producing the transgenic pig, and to a method for producing organs, from the transgenic pig, in which the immune rejection response is inhibited.

Transgenic pig in which HO-1 and TNFR1-Fc are simultaneously expressed, and method for producing same

Provided is a method for producing a cloned dog by enucleating an oocyte of a dog to produce an enucleated oocyte, transferring a somatic cell of the dog into the enucleated oocyte, carrying out electrofusion under optimized conditions to produce a nuclear transfer embryo, and transferring the nuclear transfer embryo into its surrogate mother.

Method for producing cloned dog

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Seoul, South Korea

Goo Jang