University of Colorado

Somalogic, Incorporated

Glenn Sanders

Larry M Gold

Nebojsa Janjic

Thale Jarvis

Frank B Dean

Stephan Kraemer

Evaldas Katilius

Charles S McHenry

James M Bullard

Stacie Bell

Garry Dallmann

Edgar Olivas

The present disclosure describes the isolation, identification and purification of aptamers having sufficiently high affinity and specificity to capture and immobilize intact sperm cells in the presence of female epithelial cells and other non-sperm semen components. The present disclosure also describes affinity-based methods for the detection of sperm cells in samples, including from forensic sample surrogates consisting of swab eluates containing a mixture of HeLa cells and sperm cells. The present disclosure describes methods for eluting sperm cell samples from swabs; methods for purifying sperm cells and methods for amplification and analysis of male DNA. The affinity-based system described herein is inexpensive, simple to use and easily implemented in forensic laboratories.

Method for purification and identification of sperm cells

Reconstituted bacterial replication assemblies and methods for their use are provided.

Bacterial replication systems and methods

Growing community of inventors

Boulder, CO, United States of America

Glenn Sanders