Affymetrix, Inc.

Centrillion Technology Holdings Corporation

International Business Machines Corporation

Affymax Technologies, N.v.

Centrillion Technologies Taiwan Co. Ltd.

Affyemtrix, Inc.

Glenn H McGall

Martin J Goldberg

Anthony Dale Barone

Robert G Kuimelis

Richard P Rava

Mark O Trulson

Wei Zhou

Jonathan Eric Forman

Lisa T Kajisa

Jennifer Dee Tan

Mark S Chee

Earl A Hubbell

Martin Diggelman

Ngo Quoc Nam

Maureen T Cronin

MacDonald S Morris

Nam Quoc Ngo

Lubert Stryer

James L Winkler

Charles Garrett Miyada

Virginia W Goss

R Fabian Pease

Bei-Shen Sywe

Filip Crnogorac

Guangyu Xu

Mel Yamamoto

Stephen P A Fodor

David J Lockhart

Stephen P Fodor

Handong Li

Rui Mei

Jacqueline A Fidanza

Melvin Yamamoto

Martin Diggelmann

Andrea Cuppoletti

Moti Jain

Dana Truong

Ann Maria Caviani Pease

Curtis W Frank

Thomas R Gingeras

Jody E Beecher

Vivi Truong

Claire Robinson

Zihui Chen

Vijay Singh

Jian Cao

Kyle B Cole

Glenn K Fu

Gregory Michael Wallraff

Phillip Joe Brock

Jeffrey William Jacobs

Steven A Sundberg

Christopher P Holmes

William Dinan Hinsberg

Edward L Sheldon

Jeffrey W Labadie

Hua Yang

Macdonald S Morris

Pei-Hua Wang

Christopher James Kubu

Yao-Kuang Chung

Jing Hu

Tzu-Kun Ku

Daniel E Falvey

John Robert Schullek

Justin Costa

Ying Chih Chang

Richard P Vinci

David Fujimoto

Kendall Hoff

Marc I Glazer

Garry Miyada

Michelle Smedsrud-Oval

Lin-Hsin Tu

Steven J Woodman

Eric Spence

Michelle Oval

Edward Timofeey

Andrei Mirzabekov

Rich P Rava

Bolan Li

Andrea Cupoletti

Dana Troung

Brian M Feldman

Bradley A Kreger

The present disclosure provides methods, device, and system for a hybridization assay to detect nucleic acid targets. The assay may use a probe array format with addressable microwells. Each microwell may retain a plurality of copies of one probe which may be complementary to a portion of a specific nucleic acid target. The plurality of copies of probes may be produced from a pooled library of probe sequences by amplification techniques, including, rolling circle amplification and emulsion-PCR. Detection of hybridization may rely on methods using ion-sensitive field effect transistor (ISFET) and optical detectors to detect signals indicating the presence of the nucleic acid targets.

Oligonucleotide probe array with electronic detection system

The present disclosure provides methods of sequencing polynucleotides and compounds, compositions useful for sequencing of polynucleotides. The chemical compounds include nucleotides and their analogs which possess a sugar moiety comprising a cleavable chemical group capping the 3'-OH group and a base that is attached to a label through a cleavable linker comprising a disulfide bond. In addition, both the disulfide bond and the cleavable chemical group are cleavable by a chemical reagent. Furthermore, after the disulfide bond is cleaved by the chemical reagent, there is no free thiol group linked to the base of the nucleotides according to the fragmentation reaction shown below as an example. Example compounds according to the present disclosure are shown as Formula (I):  

Disulfide-linked reversible terminators

The present disclosure relates to processes for derivatizing a surface of a substrate with a covalently bonded thin film of poly(methylsilsesquioxane)-bonded polymers as a platform for the synthesis of a biomolecule array. These processes can also be used to prepare a surface of a substrate for an in situ solid-phase synthesis of biomolecule array.

Preparation of universal spin-coatable amine-reactive surface coatings for biomolecule array fabrication

Provided herein are methods and compositions for the sequencing of long nucleic acids, such as DNA. The methods and compositions are suited for the spatial labeling and sequencing of long nucleic acid molecules.

Methods for sequencing nucleic acids

A microarray carrier assembly including a scan tray and a plurality of microarray blocks detachably disposed on the scan tray is provided. The scan tray includes a frame including an opening and a slot, and a transparent substrate covering the opening of the frame. Each of the microarray blocks includes a main body, a probe array distributed on the main body and facing towards the transparent substrate of the scan tray, and a plurality of guiding pins disposed on the main body and surrounding the probe array, wherein a top surface area of the guiding pin opposite to the main body is less than a bottom surface area of the guiding pin connected to the main body, and the guiding pins are detachably inserted into the slot of the frame of the scan tray.

Microarray carrier assembly

The present disclosure relates to processes for inverting oligonucleotide probes in an in situ synthesized array. These processes can be used to reverse the orientation of probes with respect to the substrate from 3'-bound to 5&#x2032;-bound. These processes can also be used to reduce or eliminate the presence of truncated probe sequences from an in situ synthesized array.

Probe inversion process for in situ synthesized probe arrays

Oligonucleotide probe inversion process for in situ synthesized probe arrays

Provided herein are methods and compositions for the fabrication of patterned arrays, such as nucleotide arrays. The methods and compositions are suited for the transfer and reorientation of array components.

Fabrication of patterned arrays

Provided herein are methods and compositions for coating surfaces with polymers. The methods and compositions are suited for conducting biological reactions.

Modified surfaces

The present disclosure provides methods, device, and system for wafer processing. The wafer processing apparatus uses lid dispenser to disperse at least one reagent to the surface of the wafer. Further, the wafer is positioned on top of a rotatable vacuum chuck configured to spread at least one reagent over the surface of the wafer via a centrifugal force or surface tension, thereby permitting the at least one reagent to react with an additional reagent. Further, when dispensing the at least one reagent, a separation gap between the lid dispenser and the wafer is at a predetermined distance, for example, from 50 &#x3bc;m to 2 mm.

Systems and methods to dispense and mix reagents

Silanation compositions containing a mixture of two or more silanation reagents, where at least one silanation reagent includes a functional group capable of supporting polymer synthesis and at least one silanation reagent includes no functional group capable of supporting polymer synthesis are useful in modulating the active site density and hydrolytic stability of a surface. These compositions are particularly useful in silanating a surface prior to preparation of a polymer array and provide for increased hybridization results.

Silane mixtures

The present invention relates generally to methods of sequencing of polynucleotides and compounds, compositions and kits useful for sequencing of polynucleotides. The chemical compounds include nucleotide and nucleoside analogs which possess a blocking group covalently attached to the 3' hydroxyl of the sugar moiety. The blocking group may optionally be additionally covalently attached to a linker and/or detectable label. The nucleotide analogs may be ribonucleotide or deoxyribonucleotide analogs. Methods include incorporation of the reversible terminator molecules into growing polynucleotide strands by polymerase enzymes, such as in single base sequencing methodologies utilizing sequential reversible termination techniques.

Reversible terminator molecules and methods of their use

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Mountain View, CA, United States of America

Glenn H McGall