University of Kentucky Research Foundation

Lubrizol Genetics, Inc.

Glenn B Collins

Thomas R Adams

David F Hildebrand

Santanu Dasgupta

Qingshun Li

Arthur G Hunt

Christopher Eric Lawrence

Wayne A Parrott

Lynn M Hartweck

Rebecca S Torisky

Paul A Lazzeri

An isolated gene fragment that encodes for acetate kinase, which confers disease resistance in plants is disclosed. The gene can be cloned into an expression vector to produce a recombinant DNA expression system suitable for insertion into cells to form a transgenic plant transformed with the gene fragment. A method for conferring disease resistance in plants that consists of growing plant host cells transformed with the expression system and expressing the gene conferring disease resistance to impart such resistance to host cells is also disclosed.

Use of bacterial acetate kinase and their genes for protection of plants against different pathogens

Disarmed A. tumefaciencs strain KYRT1, derived from a highly tumorigenic strain identified as A. tumefaciens strain Chry5. Disarming is accomplished by inactivation of plasmid pTiChry5 T-DNA sequences by, for example, deletion of sequences comprising the T-DNA right border. Methods of making transgenic plants using the novel A. tumefaciens strains are also provided.

KYRT1, a disarmed version of a highly tumorigenic Agrobacterium

A method for somatic embryogenesis of soybean, (Glycine max), Glycine soja and other Glycine species is provided using immature cotyledon tissue, preferably with the embryonic axis removed, comprising culturing said tissue on a medium containing auxin, preferably NAA at a concentration of at least about 15 mg/l. A further method for such somatic embryogenesis is provided wherein the culture medium contains a synergistically acting lowered carbohydrate and auxin concentration. Particularly embryogenic cells of such tissue are identified and improved maceration methods for contacting such cells with regeneration and transformation media are disclosed. Methods for transforming somatic tissue from soybean and other Glycine species are also provided. Whole, fertile, transformed plants are obtained.

Transformation, somatic embryogenesis and whole plant regeneration

Growing community of inventors

Lexington, KY, United States of America

Glenn B Collins