Menarini Silicon Biosystems, Inc.

Silicon Biosystems S.p.a.

Giuseppe Giorgini

Nicolò Manaresi

Gianni Medoro

Alex Calanca

Gerardo Perozziello

Nicolo' Manaresi

Antonio Fittipaldi

Nicolo Manaresi

The present invention relates to a method for the diagnosis of tumoural conditions and/or of the corresponding state of advance, wherein a sample from a patient comprising at least one tumour cell is obtained. According to the invention, a purified specimen of the at least one tumour cell is obtained by individually selecting and isolating single cells in a microfluidic device the purified specimen having a purity of at least 90%. On the purified specimen thus obtained there is subsequently performed a molecular analysis such as to highlight a characteristic thereof suited to enabling diagnosis.

Method for identification, selection and analysis of tumour cells

Method for the treatment of a biological sample comprising at least one cell and a liquid component; according to the method, a force is applied to the sample inserted in an inner chamber of a hollow device towards a filter which has pores with diameters from 2 nm to 1 &#x3bc;m, so that at least part of the liquid component passes through the filter and the cell remains in the inner chamber, thus obtaining a concentrated sample; the filter has a surface facing the inner chamber of less than 12.6 mm.

Devices, apparatus, kit and method for treating a biological sample

The present invention relates to a method for the diagnosis of tumoural conditions and/or of the corresponding state of advance, wherein a specimen of an organic fluid taken from the patient and having a high probability of containing at least one circulating tumor cell (CTC) or a disseminated tumor cell (CTD) is enriched in a population of CTCs or CTDs. According to the invention, at least one type of CTCs or CTDs is isolated by selecting individually single cells in a microfluidic device so to constitute a diagnostic specimen having a purity of at least 90%. On the specimen thus obtained there is subsequently performed a molecular analysis such as to highlight a characteristic thereof suited to enabling diagnosis.

A sorting chamber () for sorting first particles () from second particles () comprising: an internal chamber (), which is delimited by a base wall (), a top wall (), and a spacer (), set between the base wall () and the top wall (); an internal chamber (), which is at least partially-delimited by the base wall () and top wall (); two passages (), which set in communication the internal chamber () with the external environment; and a plurality of cavities (), which are designed to house the first particles (), are made in the base wall () and have openings () towards the internal chamber () with widths of from 4 to 6 &#x3bc;m.

Sorting chamber for microscale particles

Method for non-invasive prenatal diagnosis comprising the following steps: a. obtain a sample of an organic fluid having a high probability of containing foetal cells from a pregnant woman; b. enrich said sample of organic fluid in at least one population of cells comprising at least one type of foetal nucleated cells; c. isolate at least one cell from among said at least one type of foetal nucleated cells; d. perform a genetic analysis on said at least one cell isolated from among said at least one type of foetal nucleated cells in order to highlight at least one genetic characteristic of said at least one foetal nucleated cell suitable for permitting said diagnosis; wherein the step of isolating at least one cell from among said at least one type of foetal nucleated cells is performed by individually selecting single cells in a microfluidic device designed for said purpose.

Growing community of inventors

Padua, Italy

Giuseppe Giorgini