Biomerieux, Inc.

Giuseppe Ferorelli

Antonio Sanesi

Veronica Lucia Rossi

Massimo Galdiero

Franco Francini

Giuseppe Ubaldini

Véronica Lucia Rossi

David Jafrancesco

A machine for automatic in vitro detection of analytes, the machine being of the type including an optical reader device capable of detecting the optical response of the reaction solution to electromagnetic stimulation using a photoelectric receiver that is carried by a movable carriage of the machine and that moves under automatic control in order to bring the photoelectric receiver into various positions, each corresponding to various respective analysis zones. The photoelectric receiver forms part of a spectrometer capable of delivering a chromatic spectral decomposition of the optical response. Also provided are methods of automatic in vitro detection and/or quantification of analytes. In one method, a chromatic spectral decomposition of the optical response is acquired and two distinct optical agents are detected separately using the spectral decomposition.

Machine and method for automated in vitro analyte detection by means of chromatic spectral decomposition of an optical response

The invention relates to a system for the in vitro detection and/or quantification, by fluorometry, of at least one analyte in a sample of fluid constituting a biological material, in particular for an immunological test, including a radiation source, followed by an optical splitter for splitting the main beam into a sample-energizing beam and a reference beam, with a first photodetector means for detecting a fluorescence ray emitted by the sample and a second photodetector means for the reference beam, said system also including a generator outputting a sinusoidal carrier signal and at least one digital demodulation signal, and a digital processing means for processing, by demodulation, the signals from the two photodetector means in order to extract a fluorescence value that is characteristic of the amplitude of the fluorescence ray and a second reference value that is characteristic of the amplitude of the reference beam.

System for in vitro detection and/or quantification by fluorometry

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Florence, Italy

Giuseppe Ferorelli