Mologic Limited

Gita Parekh

Paul James Davis

James Alexander Schouten

Julie Thompson

James Schouten

Chris Dunston

Paul Davis

The present invention relates to detecting cleavage activity of an enzyme. The various aspects of the invention include an enzyme detection device, kit, method and use for detecting or measuring the presence in a test sample of the activity of an enzyme capable of cleaving a substrate. The invention also relates to indicator and binding molecules useful for carrying out the invention. The enzyme substrate contains a hidden binding site which is only revealed upon cleavage by the enzyme.

Detection of cleavage activity of an enzyme

Methods for monitoring inflammation status of a subject comprise determining levels of at least one neutrophil activation marker, or at least three markers, in urine samples taken from the subject at multiple time points, wherein increased levels of the at least one neutrophil activation marker, or at least one of the markers, in a urine sample are indicative of or predictive of an exacerbation of inflammation and/or wherein decreased levels of the at least one neutrophil activation marker, or at least one of the markers, in a urine sample following an increase are indicative or predictive of recovery from, or successful treatment of, an exacerbation of inflammation. Corresponding systems, test kits and computer programs are provided.

Monitoring inflammation status

Described herein is an enzyme detection device for use in the detection of enzyme activity in a test sample. Also provided are indicator molecules for use in the detection of enzyme activity, particularly enzyme cleavage activity, in a test sample, and to methods for detecting the presence of enzyme activity.

Enzyme detection device

Described herein is an enzyme detection device for detecting or measuring the presence in a test sample of the activity of an enzyme capable of cleaving a substrate. Also provided are methods for detecting enzyme activity, in particular the presence in a test sample of an enzyme capable of cleaving a substrate, and methods for determining the level or amount of such an enzyme in a test sample.

Growing community of inventors

Milton Keynes, United Kingdom

Gita Parekh