Council of Scientific and Industrial Research

Girish Chandra Uniyal

Rajender Singh Sangwan

Sushil Kumar

Suman Preet Singh Khanuja

Ajit Kumar Shasany

Ghulam Nabi Qazi

Madan Mohan Gupta

Rakesh Tuli

Krishan Avtar Suri

Neelam Singh Sangwan

Archana Mathur

Ajay Kumar Mathur

Mahesh Pal

Avdhesh Kumar Srivastava

Narayan Das Chaurasiya

Mahendra Panduranga Darokar

Laxmi Narayan Misra

Payare Lal

Ravoor Shankara Rao Chandrashekara

Kuppusamy Baskaran

Raghavendra Narayan Rao Kulkarni

The present invention relates to an improved process of analytical and quantitative isolation of withaferin-A from(Sanskrit: Ashwagandha, English: winter cherry) and other plants and products therefrom, said method comprising steps of selecting most appropriate extraction medium composition, with extraction solvent system consisting of a defined admixture of water and alcohol in a range of proportion of alcohol within a narrow range of (methanol, ethanol etc.) (preferably 60% alcohol and 40% water) for the dry plant material and from 60% to 80% alcohol in the alcohol-water mixture for fresh plant material, the invented extractant composition leading to better/improved yields and accurate quantitative estimations of withaferin A existent in planta or in materia, selecting effective state of the tissue particularly use of the fresh materials improving yields, providing logistic benefits of isolations and diagnosis, and according several other advantages including technological, economic, preventing contaminations etc., the said method being valuable and advantageous for standardization of botanicals, metabolomic characterization of plants particularly chemotypes, analyzing metabolic transitions under physiological, genetical, environmental and biotic perturbations.

Process for isolation of withaferin-A from plant materials and products therefrom

The invention relates to the development of a new and distinct mutant 'Dhawal' of periwinkle, , produced by chemical mutagen treatment of the seeds followed by rigorous selection in a widely cultivated variety 'Nirmal' of , said plant being stable, homozygous and produces conspicuously higher herbage and alkaloid yield.

'Dhawal', a high alkaloid producing periwinkle plant

The invention provides stable high ginsenoside-yielding callus lines of,(American Ginseng). The callus lines are useful in the industrial production of ginsenosides for use in a variety of ginseng preparations.

Stable high ginsenoside-yielding callus line of Panax quinquefolium (American ginseng) and a method for developing such stable high ginsenoside-yielding callus line

Growing community of inventors

Lucknow, India

Girish Chandra Uniyal