Applied Nanosystems B.v.

Quest International B.v.

Rijksuniversiteit Groningen

Academisch Ziekenhuis Groningen

Girbe Buist

Jan Kok

Cornelis Johannes Leenhouts

Oscar Paul Kuipers

Ranjan Ramasamy

Anton Steen

Gerard Venema

Adrianus M Ledeboer

Jan Maarten Van Dijl

Herman Groen

Hendrik Petrus Jozef Bonarius

Neeltje Margaretha Kooi

Goffe Sytse Elsinga

The invention relates to the field of immunology and vaccine development. In particular, it relates to antibodies and fragments thereof againstand to therapeutic and diagnostic uses thereof. Provided is an isolated antibody or a functional fragment thereof, which binds to an epitope of the Immunodominant Staphylococcal antigen A (IsaA), wherein said antibody comprises at least four Isa A epitope-binding CDR sequences selected from the group consisting of SEQ ID NO: 1; SEQ ID NO: 2; SEQ ID NO: 3; SEQ ID NO: 4; SEQ ID NO: 5; SEQ ID NO: 6, and functional equivalents thereof having an amino acid sequence that is at least 85% identical to an amino acid sequence of SEQ ID NO: 1-6.

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Methods for improving binding of a proteinaceous substance to cell-wall material of a Gram-positive bacterium are disclosed. The proteinaceous substance includes an AcmA cell-wall binding domain, homolog or functional derivative thereof. The method includes treating the cell-wall material with a solution capable of removing a cell-wall component such as a protein, lipoteichoic acid or carbohydrate from the cell-wall material and contacting the proteinaceous substance with the cell-wall material.

Immunization with bacterial ghost-based vaccines

The invention relates to surface display of proteins on microorganisms via the targeting and anchoring of heterologous proteins to the outer surface of cells such as yeast, fungi, mammalian, plant cells, and bacteria. The invention provides a proteinaceous substance comprising a reactive group and at least one attaching peptide including a stretch of amino acids having a sequence corresponding to at least a part of the consensus amino acid sequence listed in FIG.and further includes a method for attaching a proteinaceous substance to the cell wall of a microorganism comprising the use of the attaching peptide.

Attaching substances to microorganisms

The invention relates to surface display of proteins on micro-organisms via the targeting and anchoring of heterologous proteins to the outer surface of cells such as yeast, fungi, mammalian and plant cells, and bacteria. The invention provides a proteinaceous substance comprising a reactive group and at least one attaching peptide which comprises a stretch of amino acids having a sequence corresponding to at least a part of the consensus amino acid sequence listed in FIG.and comprises a method for attaching a proteinaceous substance to the cell wall of a micro-organism comprising the use of said attaching peptide.

Attaching substances to micro-organisms

Method to provide bacterial ghosts provided with antigens

Bacterial ghosts provided with antigens

Process for the lysis of a culture of lactic acid bacteria by means of a

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Sappemeer, Netherlands

Girbe Buist