Katholieke Universiteit Leuven

Oxford University Innovation Limited

Rijksuniversiteit Groningen

Isis Innovation Limited

Giovanni Maglia

Mikhael Soskine

John Hagan Pryce Bayley

Andrew John Heron

David Jackson Stoddart

Lakmal Nishantha Jayasinghe

Elizabeth Jayne Wallace

Kunwar Tanuj Sapra

Annemie Biesemans

Tine Brouns

Lorenzo Franceschini

Gea Schuurman-Wolters

Bert Poolman

Veerle Van Meervelt

Mariam Mohamed Abdelsattar Bayoumi

Gang Huang

Misha Soskine

Natalie Lisa Mutter

Carsten Wloka

Described herein are nanopore biosensors based on a modified cytolysin protein. The nanopore biosensors accommodate macromolecules including proteins and nucleic acids, and may additionally comprise ligands with selective binding properties.

Nanopore biosensors for detection of proteins and nucleic acids

The invention relates generally to the field of nanopores and the use thereof in various applications, such as analysis of biopolymers and macromolecules, typically by making electrical measurements during translocation through a nanopores. Provided is a system comprising a funnel-shaped proteinaceous nanopore comprising an a-helical pore-forming toxin that is a member from the actinoporin protein family, more in particular Fragaceatoxin C (FraC), a mutant FraC, a FraC paralog, or a FraC homolog.

Biological nanopores for biopolymer sensing and sequencing based on FRAC actinoporin

The invention provides a compartmentalised gel matrix comprising one or more compartments, wherein each compartment comprises a volume of hydrophobic medium and one or more aqueous droplets therein. The invention further provides a pharmaceutical formulation comprising a compartmentalised gel matrix according to the invention, a synthetic cell comprising a compartmentalised gel matrix according to the invention and a synthetic tissue comprising a compartmentalised gel matrix according to the invention.

Compartmentalised gel matrix and method of production

A method for detecting an analyte in a sample includes the steps of obtaining a nanopore sensor comprising a nanopore and a protein adaptor internalized in the lumen of the nanopore, adding a sample comprising an analyte to the cis side or the trans side of the nanopore, and measuring conductance across the nanopore. A change in conductance after adding the sample indicates the analyte is present in the sample and has bound to the protein adaptor. Nanopore sensors comprise a nanopore and a protein adaptor internalized in the lumen of the nanopore. The protein adaptor is a functional enzyme or ligand-binding protein.

Nanopores with internal protein adaptors

Provided herein relate to modified or mutant forms of cytolysin A (ClyA) and compositions comprising the same. In particular, the modified or mutant forms of ClyA permits efficient capture and/or translocation of a target negative-charged molecule or polymer through the modified or mutant ClyA nanopores at low or physiological ionic strengths. Thus, methods for using the modified or mutant forms of ClyA and compositions, for example, for characterizing a target negatively-charged analyte, e.g., a target polynucleotide, are also provided.

Modified nanopores, compositions comprising the same, and uses thereof

The invention relates to a method for sequencing a heteropolymeric target nucleic acid sequence that involves stochastic sensing. The invention also relates to a method for improving a pore for sequencing a target nucleic acid sequence by modifying one or more sites in the pore.

Method for sequencing a heteropolymeric target nucleic acid sequence

The invention relates to enhancing translocation of a charged analyte through a transmembrane protein pore. Translocation is enhanced by increasing the net opposing charge of the barrel or channel and/or entrance of the pore. The invention also relates to pores enhanced in accordance with the invention.

Methods of enhancing translocation of charged analytes through transmembrane protein pores

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Leuven, Belgium

Giovanni Maglia