Milestone S.r.l.

Addax Biosciences S.r.l.

Giovanni Bussolati

Francesco Visinoni

Michele Martinelli

Marco Bellini

The invention refers to a composition for the fixation of histological preparations, comprising a solution of glyoxal, wherefrom acids, normally present in the commercially available glyoxal, have been removed. The composition of the invention provides an optimal fixative for the preservation of structure, antigenic components and nucleic acids in tissues. The acid-free glyoxal has limited toxicity, is not considered as a carcinogenic agent and represents a valid alternative to formalin for the fixation of tissues and cells.

Acid free glyoxal as fixative for histological preparations

The invention refers to a container () for collecting, transporting and storing biological tissue samples, wherein the container () is filled with a first component () being a fixative and a second component () being a fluid, wherein the second component () has a lower specific gravity compared to the fixative (), and is immiscible with the fixative (). The second component () is stratified on top of the fixative () to form a protective film to prevent fixative fumes to escape from the container (). The invention further relates to a method for collecting, transporting and storing biological tissue samples. The method comprises the steps of: filling a first component () being a fixative in a container (), and filling a second component () being a fluid in the container () filled with the first component (), wherein the second component () has a lower specific gravity compared to the fixative () and is immiscible with the fixative () so that the second component () is stratified on top of the fixative () to form a protective film to prevent fixative fumes to escape from the container (). The method further comprises the step of placing a biological tissue sample into the first component () before or after the second component () has been filled into the container ().

Container and method for collecting, transporting and storing biological tissue samples

A process for preserving tissues is described which comprises transferring the tissues in a container and evacuating the container.

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Turin, Italy

Giovanni Bussolati