Miles Laboratories, Inc.

Giovanni Berti

William I White

Rodric H White-Stevens

Piero Fossati

Giancarlo Acquati

A composition, test device, method of making a test device and process for bilirubin-resistant determination of an analyte selected from uric acid or cholesterol in a fluid sample are disclosed. More particularly, the composition is of the type comprising means responsive to the presence of the particular analyte to be determined in the sample, a phenol and 4-aminophenazone to which resistance to interference by bilirubin is imparted by inclusion therein of reagent means comprising a ferrocyanide ion. The uric acid or cholesterol responsive means is preferably of the type which determines peroxides formed from enzymatic conversion of the analyte. The composition can optionally be incorporated with a carrier to provide a test device.

Bilirubin-resistant determination of uric acid and cholesterol

Test means, such as a composition or device, method of making a test device and process for determination of at least one oxidizing substance, such as a peroxide, are disclosed. More particularly, the contemplated test means comprises a hydrazone and 8-amino-1-napthol-5,7-disulfonic acid (Chicago acid). Further provided is a test system for the determination of a constituent in a sample, having means responsive to the presence of said constituent in said sample to produce at least one oxidizing substance and a composition for determining said at least one oxidizing substance, wherein said composition comprises a hydrazone and 8-amino-1-napthol-5,7-disulfonic acid (Chicago acid). The test system is preferably of the type which determines peroxides formed from enzymatic conversion of constituents in biological fluids. When in the form of compositions the test means can optionally be incorporated with a carrier, such as a tablet or matrix, to provide a test device. The test system is highly sensitive to low levels of body fluid constituents to be detected, while also being highly resistant to interfering reducing substances, such as ascorbic acid, often present in body fluids.

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Malgrate, Italy

Giovanni Berti