Politecnico Di Milano

Fondazione Istituto Italiano Di Tecnologia

Universitá Degli Studi Di Genova

Giorgio Tortarolo

Alberto Diaspro

Paolo Bianchini

Giuseppe Vicidomini

Federica Villa

Marco Castello

Alberto Tosi

Mauro Buttafava

Colin J R Sheppard

Simonluca Piazza

A method for operating a point laser-scanning microscope includes scanning a sample with a focused illumination laser beam; recording a plurality of images by detecting elements being configurable to an intensity mode, in which the recorded images are intensity images g(n) related to photons collected during an entire dwell time of the illumination beam on an individual position n, or to a time-resolved mode, in which the recorded images are time-resolved images g(n, t), the collected photons being discriminated based on their arrival times to individual detecting elements; calculating a fingerprint image a by summing the plurality of intensity images g(n) over all positions n; estimating shift matrices sand sfrom the intensity images g(n); reconstructing at least one of a time-resolved object function fand an intensity object function f; and visualizing at least one of a high-resolution time-resolved image fand a high-resolution intensity image f.

Time-resolved imaging method with high spatial resolution

Growing community of inventors

Genoa, Italy

Giorgio Tortarolo