Vilnius University

Gintautas Tamulaitis

Virginijus Siksnys

Migle Kazlauskiene

Thomas Fricke

Miglé Kazlauskiene

Georgij Kostiuk

Matthias Bochtler

Virginijus Šikšnys

A method of synthetizing cyclic oligoadenylates using a novel catalytic activity of a protein possessing the Palm domain, such as the Cas10 protein, and using such compounds for activation of proteins possessing the CARF doman, such as the Csm6 protein.

Regulation of CRISPR-associated rossman fold (CARF) domain containing proteins by oligoadenylates

Methods and compositions using a CRISPR-Cas Type IIIA resulting in RNA gene knockdown and knockout in an animal.

Targeted RNA knockdown and knockout by type III-A Csm complexes

A Type III-A CRISPR-Cas (StCsm) complex ofcomprising crRNA, Csm4, and Csm3 and use for cleavage of RNA bearing a nucleotide sequence complementary to the crRNA, in vitro or in vivo. Methods for site-specific cleavage/shredding of a target RNA molecule using an RNA-guided RNA endonuclease comprising a minimal complex of crRNA, Csm4, and Csm3, and methods of RNA knock-down and RNA knock-out are disclosed.

Programmable RNA shredding by the type III-A CRISPR-Cas system of

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Vilnius, Lithuania

Gintautas Tamulaitis