Dsm IP Assets B.v.

Evonik Operations Gmbh

Ginger Marie Shank

Neil Francis Leininger

Kirt Lyvell Matthews, Sr

Nasrin Tabayehnejad

Michael B Johnson

Xiao Daniel Dong

Mark Barker

Vinod Tarwade

Michael Benjamin Johnson

Kirt Lyvell Matthews

Christian Rabe

Martin Heining

Jochen Lebert

Holger Pfeifer

David Allen Tinsley

Shannon Elizabeth Ethier Resop

Michael Diehl

Georg Borchers

Mark Edward Nejako

Jurgen Haberland

Mathias Dernedde

Manfred Barz

Shannon Elizabeth Resop

Daniel Verkoeijen

Joseph W Pfeifer, Iii

John Hogan

Manfred Bärz

Stephen Cherinko

Robert Cody Kertis

Micah Needham

Marc Beiser

Mark Edward Nejako, Ii

Annika Hartmann

Brock Triplett

Negash Adugna

Vidya Pai

Jürgen Haberland

Mark Edward Nejako Ii

Sr Kirt Lyvell Matthews

Stephen Robert Cherinko

Joseph William Pfeifer

Joseph William Pfeifer Iii

Disclosed herein are processes for reducing or eliminating emulsion during the process of obtaining a microbial oil comprising one or more polyunsaturated fatty acids (PUFAs) from one or more microbial cells by washing the fermentation broth with at least 1× amount of water. Further disclosed herein is microbial oil comprising one or more PUFAs that is recovered from microbial cells by at least one process described herein.

Method of reducing emulsion by broth washing

Disclosed herein are processes for obtaining a microbial oil comprising one or more polyunsaturated fatty acids (PUFAs) from one or more microbial cells by lysing the cells to form a lysed cell composition, treating the lysed cell composition to form an oil-containing emulsion and then recovering the oil from the oil-containing emulsion. Further disclosed herein is microbial oil comprising one or more PUFAs that is recovered from microbial cells by at least one process described herein.

Processes for obtaining microbial oil from microbial cells

The current invention relates to a method of separating polyunsaturated fatty acids containing lipids from a lipids containing biomass.

Method of separating lipids from a lysed lipids containing biomass

The invention relates to the isolation of polyunsaturated fatty acids (PUFAs) from a lipids containing biomass. The main characteristics of the method are that a suspension of cells containing PUFAs is obtained and the cells are optionally lysed. The suspension is concentrated and then incubated for at least an hour at a controlled temperature while adding a specific range of base equivalents to biomass and maintaining a moderate pH. The isolation of lipid may be performed in the absence of organic solvents and without the addition of salt.

Method of isolating lipids from a lipids containing biomass

Disclosed herein are processes for obtaining a microbial oil comprising one or more polyunsaturated fatty acids (PUFAs) from one or more microbial cells by lysing the cells to form a lysed cell composition and then recovering the oil from the lysed cell composition. Further disclosed herein is microbial oil comprising one or more PUFAs that is recovered from microbial cells by at least one process described herein.

Disclosed herein are processes for obtaining a microbial oil comprising one or more polyunsaturated fatty acids (PU-FAs) from one or more microbial cells by lysing the cells to form a lysed cell composition and then recovering the oil from the lysed cell composition. Further disclosed herein is microbial oil comprising one or more PUFAs that is recovered from microbial cells by at least one process described herein.

Processes are disclosed for obtaining a lipid from a cell by lysing the cell to form a lysed cell composition, adding an acid to lower the pH of the lysed cell composition, and demulsify the lysed cell composition, and obtaining the lipid from the demulsified lysed cell composition. Also disclosed are lipids prepared by the processes of the present invention wherein the lipids have a particular anisidine value, peroxide value, and/or phosphorus content.

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Winchester, KY, United States of America

Ginger Marie Shank