University of Georgia Research Foundation, Inc.

Gina L Lipscomb

Janet Westpheling

Michael W W Adams

Joel Andrew Farkas

Provided herein are methods for transforming awith a polynucleotide. In one embodiment, the method includes contacting awith a polynucleotide under conditions suitable for uptake of the polynucleotide by the, and identifying transformants at a frequency of, for instance, at least 10transformants per microgram DNA. Also provided are isolatedhaving the characteristics ofCOM1, and plasmids that include an origin of replication that functions in a. The plasmid is stable in a recipientwithout selection for more than 100 generations and is structurally unchanged after replication infor more than 100 generations.

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Athens, GA, United States of America

Gina L Lipscomb