United States of America, As Represented by the Secretary of Agriculture

Institut Pasteur

Centre National De La Recherche Scientifique

Institut National De Recherche Pour L'agriculture, L'alimentation Et L'environnement

Institut National De La Recherche Agronomique (inra)

Gilles Etienne

Sylvie Bay

John P Bannantine

Franck Biet

Gerard Tiraby

Daniel Drocourt

Elise Armau

The present invention is directed to an isolated synthetic tripeptide of formula H-D-Phe-N-Methyl-L-Val-L-Ala-OMe (SEQ ID NO: 1), or a derivative thereof, and to the corresponding lipotripeptides, which are specific tosubsp.(Map) S-type strain, as well as derivatives and conjugates thereof. The invention also concerns the use of these antigens in different methods and tests for detecting Map infection, especially by detecting humoral response and cell mediated response of infected animals. The invention is also directed to a genetic signature of Map and a mass spectrometry and NMR spectroscopy signature of Map presence or infection.

Antigenic tripeptides derived fromsubsp.S-type strains, derivatives and uses thereof

The present invention is directed to an isolated synthetic tripeptide of formula H-D-Phe-N-Methyl-L-Val-L-Ala-OMe (SEQ ID NO:1), or a derivative thereof, and to the corresponding lipotripeptides, which are specific tosubsp.(Map) S-type strain, as well as derivatives and conjugates thereof. The invention also concerns the use of these antigens in different methods and tests for detecting Map infection, especially by detecting humoral response and cell mediated response of infected animals. The invention is also directed to a genetic signature of Map and a mass spectrometry and NMR spectroscopy signature of Map presence or infection.

Antigenic tripeptides derived fromsubsp.s-type strains, derivatives and uses thereof

A method of transforming eukaryotic or prokaryotic hosts sensitive to an antibiotic of the phleomycin family to confer resistance to the antibiotic is disclosed in which a phleomycin resistance gene is used as a selectable marker.

Application of antibiotics of the phleomycin family as selection agent

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Toulouse, France

Gilles Etienne