Alteogen, Inc.

Cj Healthcare Corporation

The Green Cross Corporation

Gil Bu Kang

Sang Hyun Lee

Soon Jae Park

Ki-Yong Kim

Young Joon Park

Jee Won Ahn

Dong Eok Lee

Ki Wan Kim

Hyoung Taek Lim

Sang Mee Lee

Chi Hye Park

Ji Hyun Moon

Yoon Jung Lee

Hye Shin Chung

Hoo Keun Oh

Chung Min Lee

Won Jeong Lee

Sung Yoo Cho

Chang Bong Jun

Dae-Eun Kang

Jihye Lee

Seol Young Yoo

Soo-Kwang Kim

The present invention relates to a long-acting human growth hormone NexP-hGH protein and its production method. More specifically, it relates to a specific isoform of long-acting human growth hormone NexP-hGH protein in which human growth hormone is fused with a highly glycosylated alpha-1 antitrypsin mutant whereby long-acting properties in vivo are increased. The present invention also relates to a high-purity purification method for NexP-hGH, which includes the steps of: (a) carrying out anion-exchange resin chromatography on a biological emulsion comprising NexP-hGH in which human growth hormone is fused with an alpha-1 antitrypsin mutant; (b) carrying out hydrophobic resin chromatography on the biological emulsion comprising NexP-hGH, or on the eluate produced in step (a); and (c) carrying out affinity chromatography, entailing packing with a resin to which anti-alpha-1 antitrypsin antibody fragments are attached, on the biological emulsion comprising NexP-hGH and on the eluate produced in step (a) or step (b).

Method of purifying a long-acting human growth hormone

Growing community of inventors

Busan, South Korea

Gil Bu Kang