Pioneer Hi-bred International, Inc.

Renagade Therapeutics Management Inc.

Giedrius Gasiunas

Joshua K Young

Virginijus Siksnys

Muthusamy Jayaraman

Andrew Mark Cigan

Vladimir Presnyak

Zhenglin Hou

Alim Ladha

Tautvydas Karvelis

Elisabeth Narayanan

The present disclosure provides methods and compositions comprising Cas TypeV programmable nucleases and lipid nanoparticles capable of delivering the Cas TypeV programmable nucleases and genome editing systems comprising same.

Gene editing components, systems, and methods of use

Compositions and methods are provided for novel Cas9 orthologs, including, but not limiting to, novel guide polynucleotide/Cas9 endonucleases complexes, single or dual guide RNAs, guide RNA elements, and Cas9 endonucleases. The present disclosure also describes methods for creating a double strand break in a target polynucleotide, methods for genome modification of a target sequence under various in vivo and in vitro conditions, in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell. Also provided are nucleic acid constructs and cells having a modified target site or altered polynucleotide of interest produced by the methods described herein.

Cas9 orthologs

Compositions and methods are provided for rapid characterization of Cas endonuclease systems and the elements comprising such systems, including, but not limiting to, rapid characterization of PAM sequences, guide RNA elements and Cas endonucleases. Type II Cas9 endonuclease systems originating fromMlc3,DSM 15814,SL4,JCM 14932,sp. SCADC,DSM 20210,NX02,DSM 16811,are described herein. The present disclosure also describes methods for genome modification of a target sequence in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell.

Rapid characterization of Cas endonuclease systems, PAM sequences and guide RNA elements

Growing community of inventors

Vilnius, Lithuania

Giedrius Gasiunas