Agency for Science, Technology and Research

Gideon Ho

Lang Zhuo

Saravana Kumar

A method is disclosed involving detecting the expression of a fluorescent protein of interest in an animal, wherein the animal is a transgenic animal having in its genome nucleic acid encoding said fluorescent protein operably linked to promoter nucleic acid from a protein that is normally expressed in the nervous system of the animal, the method comprising the step of non-invasively detecting fluorescence from said protein when expressed in said animal.

Non-invasive, in vivo fluorescent imaging of the nervous system in whole living animal

A method is provided for averaging a sequence of image frames. A noise-reducing filter is applied to the image frames to generate filtered frames. A deconvolution filter is applied to the filtered frames to generate corresponding deconvolved frames. The filtered frames are transformed by an affine transformation to align them, generating aligned frames. The aligned frames are motion corrected by non-linear transformation based on intensity rank matching, generating a sequence of motion-corrected frames. The motion-corrected frames are averaged to generate a resultant frame.

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Singapore, Singapore

Gideon Ho