Institute of Nuclear Energy Research, Atomic Energy Council, Executive Yuan

Atomic Energy Council-institute of Nuclear Energy Research

National Pingtung University of Science & Technology

Gia-Luen Guo

Wen-Song Hwang

Jia-Baau Wang

Chiung-Fang Huang

Ting-Hsiang Lin

Yi-Feng Jiang

Yo-Chia Chen

Teng-Chieh Hsu

Chun-An Wang

Jeng-Chen Liu

Hau-Yu Wang

Sheng-Hsin Chou

Wei-Hsi Chen

Wen-Heng Chen

A preparation method conducive to enhancing enzymatic activity of cellulase includes the steps of preparing a first inducer, preparing preculture hyphae, producing enzymes by a single strain, feeding a second inducer, and producing enzymes by co-culture strains. Main ingredients of the first inducer and the second inducer are cellulose and lactose, respectively. The enzymatic activity of the cellulase produced is enhanced by induction of cellulose and lactose and co-culture ofand

Preparation method conducive to enhancing enzymatic activity of cellulase

A method for producing xylitol by fermentation of lignocellulosic hydrolysates without detoxification is provided. By using the originally isolated yeastsp., xylose can be effectively converted into xylitol. The invention also provides thestrain having high furfural tolerance, and is capable to produce xylitol from various types of non-detoxified lignocellulosic hydrolysates, in which the overall utilization of xylose in hydrolysate can reach over 95%.

Method for producing xylitol from lignocellulosic hydrolysates without detoxification

A cellulose hydrolase and a gene thereof are obtained by screening a cDNA genomic library constructed withsp. Y102. The gene is 1071 base pairs long and comprises an open reading frame (ORF) for producing the cellulose hydrolase comprising 357 amino acids by translation. A transformed cell and a carrier carrying the gene are introduced. The gene is transferred toby transformation, such thatcan acquire activity of decomposing CMC, beta-glucan, and xylan. The cellulose hydrolase is multifunctional and is capable of decomposing cellubiose and directly decomposing fiber into glucose.

Cellulose hydrolase and gene thereof

A method for culturing the yeast for enhancing pentitol production is provided. The yeast cultured according to the present disclosure is. Application of the yeast in pentitol production by fermention of the lignocellulosic hydrolysate or the xylose-to-pentitol production yield can be enhanced 3 to 6 times from the non-detoxified or the overliming-processed lignocellulosic hydrolysate.

Method of cultivating yeast for enhancing pentitol production

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Taipei County, Taiwan

Gia-Luen Guo